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CHRM1 Antibody (PA5-95151) in ICC/IF
Immunofluorescence analysis of CHRM1 in A431 cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with CHRM1 polyclonal antibody (Product # PA5-95151) at a 2 µg/1x10^6 cells dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution and DAPI.
Product Details
PA5-95151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human muscarinic Acetylcholine Receptor 1 (303-317aa KMPMVDPEAQAPTKQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806956
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor, muscarinic 1; AChR M1; cholinergic receptor; Cholinergic receptor, muscarin 1; cholinergic receptor, muscarinic 1; M1 mAChR; m1 muscarinic acetylcholine receptor protein; MGC30125; Muscarinic acetylcholine receptor M1
Gene Aliases: Chrm-1; CHRM1; HM1; M1; M1R
UniProt ID: (Human) P11229, (Mouse) P12657, (Rat) P08482
Entrez Gene ID: (Human) 1128, (Mouse) 12669, (Rat) 25229
regulation of vascular smooth muscle contraction
cellular protein modification process
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
protein kinase C-activating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway
G-protein coupled acetylcholine receptor signaling pathway
synaptic transmission, cholinergic
neuromuscular synaptic transmission
nervous system development
cell proliferation
positive regulation of cell proliferation
regulation of locomotion
positive regulation of ion transport
saliva secretion
cognition
positive regulation of intracellular protein transport
sensory perception of chemical stimulus
regulation of ion transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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