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Invitrogen
CHRNA3 Polyclonal Antibody
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FIGURE: 1 / 11
CHRNA3 Antibody (PA5-116458) in IHC (P)
Immunohistochemical analysis of CHRNA3 in a paraffin-embedded human tonsil carcinoma tissue using a CHRNA3 polyclonal antibody (Product #PA5-116458). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:... View More
Product Details
PA5-116458
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within rat CHRNA3 aa 73-122.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive controls: SH-SY5Y cell lysate, Hela cell lysate, human stomach tissue lysate, rat brain tissue lysate, rat testis tissue, human liver carcinoma tissue, human colon carcinoma tissue, human skin tissue, human breast carcinoma tissue, human kidney tissue, human esophagus tissue, human small intestine tissue, human tonsil carcinoma tissue, SH-SY5Y.
Target Information
CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholine receptor; Acetylcholine receptor alpha 3 (neuronal nicotine); alpha 3; cetylcholine receptor alpha 3 (neuronal nicotine); cholinergic receptor; cholinergic receptor nicotinic alpha polypeptide 4; cholinergic receptor, nicotinic alpha 3; cholinergic receptor, nicotinic, alpha 3 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 3; cytotransmitter gated ion channel; nAChR alpha 3; Neuronal acetylcholine receptor subunit alpha-3; neuronal nicotinic acetylcholine receptor, alpha 3 subunit; neuronal nicotinic acetylcholine receptor, alpha3 subunit; unnamed protein product
Gene Aliases: (a)3; A730007P14Rik; Acra-3; Acra3; BAIPRCK; CHRNA3; LNCR2; NACHRA3; PAOD2
UniProt ID: (Human) P32297, (Mouse) Q8R4G9
Entrez Gene ID: (Human) 1136, (Mouse) 110834, (Rat) 25101
transmembrane signaling receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
protein binding
ligand-gated ion channel activity
acetylcholine receptor activity
acetylcholine-gated cation channel activity
acetylcholine binding
acetylcholine-activated cation-selective channel activity
drug binding
protein heterodimerization activity
ligand-gated ion channel
ion transport
regulation of smooth muscle contraction
signal transduction
synaptic transmission, cholinergic
neuromuscular synaptic transmission
nervous system development
locomotory behavior
regulation of acetylcholine secretion, neurotransmission
ion transmembrane transport
response to nicotine
behavioral response to nicotine
regulation of membrane potential
regulation of dendrite morphogenesis
membrane depolarization
excitatory postsynaptic potential
synaptic transmission involved in micturition
acetylcholine receptor signaling pathway
presynaptic modulation of chemical synaptic transmission
response to acetylcholine
transport
cation transport
regulation of muscle contraction
activation of transmembrane receptor protein tyrosine kinase activity
chemical synaptic transmission
response to drug
protein heterooligomerization
cation transmembrane transport
heart development
response to inorganic substance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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