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Invitrogen
CHRNA3 Polyclonal Antibody
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FIGURE: 1 / 1
CHRNA3 Antibody (PA5-95837) in WB
Western blot analysis of CHRNA3 in extracts of various cell lines. Samples were incubated with CHRNA3 Polyclonal antibody (Product # PA5-95837) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-95837
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 32-240 of human CHRNA3 (NP_0007342)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SEAEHRLFER LFEDYNEIIR PVANVSDPVI IHFEVSMSQL VKVDEVNQIM ETNLWLKQIW NDYKLKWNPS DYGGAEFMRV PAQKIWKPDI VLYNNAVGDF QVDDKTKALL KYTGEVTWIP PAIFKSSCKI DVTYFPFDYQ NCTMKFGSWS YDKAKIDLVL IGSSMNLKDY WESGEWAIIK APGYKHDIKY NCCEEIYPDI TYSLYIRRL; Positive Samples: SH-SY5Y, HepG2, Mouse brain, Rat liver; Cellular Location: Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Target Information
CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholine receptor; Acetylcholine receptor alpha 3 (neuronal nicotine); alpha 3; cetylcholine receptor alpha 3 (neuronal nicotine); cholinergic receptor; cholinergic receptor nicotinic alpha polypeptide 4; cholinergic receptor, nicotinic alpha 3; cholinergic receptor, nicotinic, alpha 3 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 3; nAChR alpha 3; Neuronal acetylcholine receptor subunit alpha-3; neuronal nicotinic acetylcholine receptor, alpha 3 subunit; neuronal nicotinic acetylcholine receptor, alpha3 subunit
Gene Aliases: (a)3; A730007P14Rik; Acra-3; Acra3; CHRNA3; LNCR2; NACHRA3; PAOD2
UniProt ID: (Human) P32297, (Mouse) Q8R4G9
Entrez Gene ID: (Human) 1136, (Mouse) 110834, (Rat) 25101
acetylcholine-activated cation-selective channel activity
drug binding
ligand-gated ion channel activity
acetylcholine receptor activity
acetylcholine binding
protein heterodimerization activity
ion channel activity
extracellular ligand-gated ion channel activity
protein binding
ligand-gated ion channel
ion transport
regulation of smooth muscle contraction
signal transduction
activation of transmembrane receptor protein tyrosine kinase activity
synaptic transmission, cholinergic
nervous system development
heart development
locomotory behavior
response to inorganic substance
regulation of acetylcholine secretion, neurotransmission
behavioral response to nicotine
regulation of membrane potential
response to drug
regulation of dendrite morphogenesis
protein heterooligomerization
excitatory postsynaptic potential
synaptic transmission involved in micturition
cation transmembrane transport
transport
cation transport
regulation of muscle contraction
chemical synaptic transmission
response to nicotine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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