Invitrogen

CHRNA6 (extracellular) Polyclonal Antibody

Product Image Gallery

CHRNA6 (extracellular) Antibody in Western Blot (WB) — Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates with CHRNA6 (extracellular) polyclonal antibody (Product # PA5-77504) using a dilution of 1:1000.

CHRNA6 (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of CHRNA6 (extracellular) in immersion-fixed, free floating, frozen rat brain. A) Samples were probed with CHRNA6 (extracellular) polyclonal antibody (Product # PA5-77504) using a dilution of 1:100, and incubated with DAPI (red). B) Stained striatal matrix and patches and the overlying corpus callosum (blue).

CHRNA6 (extracellular) Antibody in Immunocytochemistry (ICC) — Immunocytochemistry analysis of CHRNA6 (extracellular) in rat PC12 pheochromocytoma cells. A) Samples were probed with CHRNA6 (extracellular) polyclonal antibody (Product # PA5-77504) at a dilution of 1:50, and incubated with goat-anti-rabbit-AlexaFluor-594. B) Live view cell image. C) Merged images of Panels A and B.

CHRNA6 (extracellular) Antibody — (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRalpha6

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000-1:2000

Immunohistochemistry (IHC)

1:1000-1:2000

Immunocytochemistry (ICC)

1:50

Immunofluorescence (IF)

1:50

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRalpha6

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2735652

Product Specific Information

Product is shipped at room temperature as a lyophilized powder and should be stored at -20C upon receipt.

Reconstitute in 50 ul deionized water.

Target Information

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acetylcholine receptor; acetylcholine receptor alpha 6 neural; alpha6 nAChR; cholinergic receptor; cholinergic receptor, nicotinic, alpha polypeptide 6; Neuronal acetylcholine receptor subunit alpha-6; nicotinic acetylcholine receptor subunit alpha 6

Gene Aliases: Acra6; Chrna6; Nica6

UniProt ID: (Rat) P43143, (Mouse) Q9R0W9

Entrez Gene ID: (Rat) 81721, (Mouse) 11440

Molecular Function: GABA receptor acetylcholine receptor ion channel ligand-gated ion channel receptor transporter