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CHRNB2 Antibody (PA5-119689) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of CHRNB2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CHRNB2 Polyclonal Antibody (Product # PA5-119689) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
PA5-119689
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within rat CHRNB2 aa 50-150
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913262
Product Specific Information
Positive Control: Jurkat cell lysates, A549, SH-SY5Y, rat spinal cord tissue, mouse brain tissue.
Subcellular Location: Postsynaptic cell membrane, cell membrane.
Target Information
Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor beta 2 neural; acetylcholine receptor, nicotinic, beta 2 (neuronal); beta2 human neuronal nicotinic acetylcholine receptor; cholinergic receptor; cholinergic receptor nicotinic beta polypeptide 2; cholinergic receptor, nicotinic beta 2; cholinergic receptor, nicotinic, beta 2 (neuronal); cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal); cholinergic receptor, nicotinic, beta subunit 2; N-alpha 1; Neuronal acetylcholine receptor non-alpha-1 chain; Neuronal acetylcholine receptor subunit beta-2; neuronal nicotinic acetylcholine receptor beta 2
Gene Aliases: [b]2-nAchR; Acrb-2; Acrb2; C030030P04Rik; CHRNB2; EFNL3; nAChRB2
UniProt ID: (Human) P17787, (Rat) P12390, (Mouse) Q9ERK7
Entrez Gene ID: (Human) 1141, (Rat) 54239, (Mouse) 11444
acetylcholine-activated cation-selective channel activity
protein binding
drug binding
ligand-gated ion channel activity
acetylcholine receptor activity
acetylcholine binding
protein heterodimerization activity
ion channel activity
extracellular ligand-gated ion channel activity
ligand-gated ion channel
action potential
conditioned taste aversion
response to hypoxia
ion transport
calcium ion transport
smooth muscle contraction
signal transduction
synaptic transmission, cholinergic
neuromuscular synaptic transmission
visual perception
sensory perception of sound
learning
memory
locomotory behavior
associative learning
visual learning
regulation of dopamine secretion
sensory perception of pain
vestibulocochlear nerve development
optic nerve morphogenesis
lateral geniculate nucleus development
central nervous system projection neuron axonogenesis
positive regulation of B cell proliferation
regulation of synaptic transmission, dopaminergic
positive regulation of synaptic transmission, dopaminergic
positive regulation of dopamine secretion
response to nicotine
behavioral response to nicotine
social behavior
regulation of dopamine metabolic process
B cell activation
response to cocaine
regulation of circadian sleep/wake cycle, REM sleep
regulation of circadian sleep/wake cycle, non-REM sleep
response to ethanol
negative regulation of action potential
regulation of dendrite morphogenesis
neurological system process
cognition
protein heterooligomerization
membrane depolarization
regulation of synapse assembly
synaptic transmission involved in micturition
cation transmembrane transport
central nervous system neuron axonogenesis
regulation of membrane potential
transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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