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Agrisera
CHS/Chalcone synthase Polyclonal Antibody
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FIGURE: 1 / 1
CHS/Chalcone synthase Antibody (AS12-2615) in WB
0.5 to 7 µg of protein from Arabidopsis thaliana Col 0 leaf tissue, extracted with Agrisera PEB protein extraction buffer 1 X were separated on 12% SDS-PAGE using tank (Bio-Rad system) to transfer to nitrocellulose membrane during 1 hour. Blots were blocked with BSA (Sigma) for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with... View More
Product Details
AS12-2615
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CHS.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Solanum lycopersicum
Target Information
Chalcone synthase is a plant-encoded, predominantly cytosolic enzyme that can associate with the cytoplasmic face of the endoplasmic reticulum as part of phenylpropanoid/flavonoid metabolon assemblies, positioning it near upstream phenylpropanoid enzymes while acting on CoA-linked substrates. Structurally, it is a soluble type III polyketide synthase (thiolase-fold) that functions as a homodimer, with each monomer contributing an active-site cavity that binds a starter acyl-CoA and iteratively extends it with malonyl-CoA-derived two-carbon units before cyclization. Catalysis relies on a conserved active-site nucleophile Cys164 and key residues that support decarboxylation/condensation chemistry and intermediate control (including His303 and Asn336, with additional "gatekeeper" residues shaping chain-length and cyclization outcomes), enabling formation of a tetraketide intermediate from one p-coumaroyl-CoA plus three malonyl-CoA equivalents that cyclizes to a chalcone product. Biologically, chalcone synthase catalyzes the first committed step into flavonoid and isoflavonoid biosynthesis, thereby underpinning production of pigments (e.g., anthocyanins), signaling and UV-protective metabolites, and antimicrobial/defense-associated compounds; its expression is commonly induced by developmental cues and stresses such as UV exposure and pathogen challenge, linking enzyme activity to adaptive secondary metabolism and plant resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CHS; naringenin-chalcone synthase
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