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CHST4 Antibody (PA5-47752) in WB
Western blot analysis of CHST4 in bEnd.3 mouse endothelioma cell line. Samples were incubated in CHST4 polyclonal antibody (Product # PA5-47752) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant mouse Carbohydrate Sulfotransferase 4/CHST4 Arg30-Gly388
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576789
Product Specific Information
In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) CHST4 is observed and less than 5% cross-reactivity with recombinant mouse CHST5 and rhCHST6 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carbohydrate sulfotransferase 4; galactose/N-acetylglucosamine/N-acetylglucosamine; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST-2; GST-3; HEC-GlcNAc6ST; high endothelial cell GlcNAC-6-sulphotransferase; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2
Gene Aliases: Chst4; Gn6st-2; GST-3; Gst3; HEC-GlcNAc6ST
UniProt ID: (Mouse) Q9R1I1
Entrez Gene ID: (Mouse) 26887
N-acetylglucosamine 6-O-sulfotransferase activity
N-acetylgalactosamine 4-O-sulfotransferase activity
heparan sulfate 2-O-sulfotransferase activity
sulfotransferase activity
HNK-1 sulfotransferase activity
transferase activity
heparan sulfate 6-O-sulfotransferase activity
trans-9R,10R-dihydrodiolphenanthrene sulfotransferase activity
1-phenanthrol sulfotransferase activity
3-phenanthrol sulfotransferase activity
4-phenanthrol sulfotransferase activity
trans-3,4-dihydrodiolphenanthrene sulfotransferase activity
9-phenanthrol sulfotransferase activity
2-phenanthrol sulfotransferase activity
phenanthrol sulfotransferase activity
1-hydroxypyrene sulfotransferase activity
galactose 3-O-sulfotransferase activity
proteoglycan sulfotransferase activity
cholesterol sulfotransferase activity
hydroxyjasmonate sulfotransferase activity
transferase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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