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Invitrogen
CHST4 Polyclonal Antibody
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FIGURE: 1 / 1
CHST4 Antibody (PA5-48076) in WB
Western blot analysis of CHST4 in SW13 human adrenal cortex adenocarcinoma cell line. Samples were incubated in CHST4 polyclonal antibody (Product # PA5-48076) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-48076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human Sulfotransferase 4/CHST4 Leu22-Gln286
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CHST4 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and rhCHST6 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; Carbohydrate sulfotransferase 4; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylglucosamine; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST-2; gn6st-2; GST-3; HEC-GlcNAc6ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2; unnamed protein product
Gene Aliases: CHST4; GlcNAc6ST2; GST3; HECGLCNAC6ST; LSST
UniProt ID: (Human) Q8NCG5
Entrez Gene ID: (Human) 10164
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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