|Tested species reactivity||Mouse, Rat, Dog, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to a region within residues 1500-1608 of the human Capicua protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2-5 µg/mg lysate|
|Western Blot (WB)||1:1000-1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
By Western blot, PA1-46018 is specific for the ~250 kDa CIC protein.
CIC (~164 kDa) interacts with Ataxin 1 (~87 kDa).
This may explain the band at 250 kDa (complex).Suggested positive control: Hela whole cell extract.
CIC, a human homolog of Drosophila capicua, encodes a high mobility group box transcription factor and is fused to a double homeodomain gene DUX4 as a result of a recurrent chromosomal translocation. It is involved in epidermal growth factor receptor (EGFR) signaling, developmental patterning, and cell fate. In humans, CIC may be involved in granule cell lineage, cerebellar development, and medulloblastoma tumorigenesis. Recent evidence indicates that CIC is involved in Spinocerebellar Ataxia Type 1 (SCA-1) where it interacts directly with the product of the SCA-1 gene Ataxin-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.