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CIITA Polyclonal Antibody
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FIGURE: 1 / 1
CIITA Antibody (100-401-249) in WB
Western blot of Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by western blot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA. For transfection, Fugene 6 (Roche) was used according to the manufacturers instructions for a 6-well plate format. Cells were lysed 24 h post-transfection in 200 µL of 1x SDS-sample buffer, heated at 96C for 5, and vortexed for 30 sec. Samples (10 µL each) were separated on ... View More
Product Details
100-401-249
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.
Conjugate
Form
Liquid
Concentration
90 mg/mL
Amount
9 mg
Storage buffer
whole serum
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
0
Target Information
CIITA contains an acidic transcriptional activation domain, four LRRs (leucine-rich repeats) and a GTP binding domain. It is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. CIITA also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIITA; MH NLRA; MHC class II transactivator; MHC class II transactivator type I; MHC class II transactivator type III; NLR family, acid domain containing; nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
Gene Aliases: C2TA; CIITA; CIITAIV; MHC2D1; MHC2TA; NLRA
UniProt ID: (Human) P33076
Entrez Gene ID: (Human) 4261
nucleotide binding
DNA binding
transcription coactivator activity
transcription corepressor activity
protein serine/threonine kinase activity
protein binding
ATP binding
GTP binding
kinase activity
transferase activity
transferase activity, transferring acyl groups
histone deacetylase binding
macromolecular complex binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
protein serine kinase activity
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
immune response
negative regulation of collagen biosynthetic process
response to interferon-gamma
positive regulation of MHC class I biosynthetic process
positive regulation of MHC class II biosynthetic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of viral entry into host cell
response to antibiotic
interferon-gamma-mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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