Invitrogen

CITED2 Polyclonal Antibody

View all (6) CITED2 antibodies

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CITED2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CITED2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114719). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

CITED2 Antibody in Western Blot (WB) — Western blot analysis of CITED2 in HeLa cells lysates (left lane: treated with antigen-specific peptide). Samples were incubated with polyclonal antibody (Product # PA5-114719).

CITED2 Antibody in Western Blot (WB) — Western blot analysis of CITED2 in P19 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-114719).

Product Details

PA5-114719

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CITED2(Accession Q99967), corresponding to amino acid residues G143-G172.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2899355

Target Information

The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of HIF1a to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Three transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cbp/p300-interacting transactivator 2; Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2; Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2; melanocyte specific gene 2; melanocyte-specific gene 1-related gene 1; MRG-1; MSG-related protein 1; MSG1-related gene 1; P35srj

Gene Aliases: AI835299; ASD8; CITED2; ER154-like; MRG-1; MRG1; Msg2; P35SRJ; VSD2

UniProt ID: (Human) Q99967, (Mouse) O35740

Entrez Gene ID: (Human) 10370, (Rat) 114490, (Mouse) 17684

Molecular Function: transcription cofactor

negative regulation of transcription from RNA polymerase II promoter response to hypoxia liver development outflow tract morphogenesis regulation of organ formation transcription, DNA-templated determination of left/right symmetry heart development sex determination cell proliferation positive regulation of gene expression negative regulation of gene expression positive regulation of cell-cell adhesion cell differentiation negative regulation of cell migration positive regulation of transforming growth factor beta receptor signaling pathway response to fluid shear stress positive regulation of peroxisome proliferator activated receptor signaling pathway adrenal cortex formation negative regulation of apoptotic process response to estrogen positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter spleen development ventricular septum morphogenesis embryonic heart tube left/right pattern formation left/right pattern formation negative regulation of transcription from RNA polymerase II promoter in response to hypoxia left/right axis specification nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry positive regulation of male gonad development blood vessel development vasculogenesis branching involved in ureteric bud morphogenesis in utero embryonic development kidney development trophectodermal cell differentiation neural tube formation neural tube closure embryonic placenta development vasculature development heart looping morphogenesis of an epithelium lens morphogenesis in camera-type eye hematopoietic progenitor cell differentiation leukocyte differentiation endocardial cushion development ventricular septum development regulation of transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway central nervous system development peripheral nervous system development cell aging male gonad development response to mechanical stimulus cranial nerve morphogenesis adrenal gland development granulocyte differentiation skeletal muscle cell differentiation decidualization thymus development embryonic camera-type eye morphogenesis erythrocyte development embryonic process involved in female pregnancy bone morphogenesis cardiac septum morphogenesis pulmonary artery morphogenesis cardiac neural crest cell development involved in heart development determination of heart left/right asymmetry cellular response to growth factor stimulus regulation of nucleic acid-templated transcription regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development

Process(es)

RNA polymerase II transcription coactivator activity RNA polymerase II transcription corepressor activity chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity transcription corepressor activity protein binding histone acetyltransferase binding LBD domain binding RNA polymerase II activating transcription factor binding molecular_function SMAD binding transcription cofactor activity