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Invitrogen
CK1 alpha Polyclonal Antibody
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FIGURE: 1 / 8
CK1 alpha Antibody (PA5-17536) in WB
Western blot was performed using anti-CK1 Polyclonal Antibody (Product # PA5-17536) and a 37 kDa band corresponding to CK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK 293 (Lane 1), HeLa (Lane 2), HCT 116 (Lane 3), MCF-7 (Lane 4) and A549 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the pri... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acid residues near the middle of human casein kinase I-alpha 1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
46 µg/mL
Amount
4.6 µg
Purification
Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/mL BSA, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
CNSK1A1 (CK1 alpha 1) is a member of the casein kinase I (CKI) gene family, which encodes serine/threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric, ranging from 25 to 55 kD, and are found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Casein kinase I isoform alpha; casein kinase I-alpha; CK I alpha; CK-I alpha; CK1; CKI alpha; CKI-alpha; CSNK1A1/CK1; OTTHUMP00000224000; OTTHUMP00000224001; OTTHUMP00000224003; YIL035C
Gene Aliases: 2610208K14Rik; 4632404G05Rik; 5430427P18Rik; CK1a; Csnk1a; CSNK1A1
UniProt ID: (Rat) P97633, (Mouse) Q8BK63
Entrez Gene ID: (Rat) 113927, (Mouse) 93687
magnesium ion binding
glycoprotein binding
protein kinase activity
protein serine/threonine kinase activity
ATP binding
peptide binding
phosphoprotein binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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