Invitrogen

CK2 beta Polyclonal Antibody

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CK2 beta Antibody — Figure 6. CK2 directly interacts with m18 and represses HDAC function. ( A ) Immunoprecipitation (IP) of m18 was performed in lysates of cells expressing m18-3xFlag or empty 3xFlag vector, and the products were analyzed for FLAG and CK2beta by western blot. Data are representative of three independent experiments. ( B ) Mouse fibroblasts were treated with CK2 inhibitor TBBt in conjunction with zVAD or zVAD alone and analyzed for RAE-1 expression by flow cytometry. Data is representative of three independent experiments. ( C ) Representative image of fibroblasts expressing m18-RFP and stained for HDAC3 pS424. ( D ) Quantification of HDAC3 pS424 levels in cells expressing m18-RFP or RFP control vector from compared to non-transfected controls in same field of view. Red bars are representative of mean+-SEM. Data are representative of three independent experiments. ****p<0.00005. n.s., not significant. (Student and apos;s T-test). DOI: http://dx.doi.org/ Figure 6--figure supplement 1. m18 expression does not change HDAC3 levels. ( A ) Representative picture of HDAC3 staining in m18-RFP transfected cells. ( B ) Nuclear staining of HDAC3 was quantified in FIJI. Data are representative of mean+-SEM. Data are representative of three independent experiments. n.s., Not significant (Students T-test). DOI: http://dx.doi.org/

CK2 beta Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Casein Kinase 2 beta in paraformaldehyde-fixed HeLa cells using a Casein Kinase 2 beta polyclonal antibody (Product # PA5-27416) at a 1:500 dilution.

CK2 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Casein Kinase 2 beta in the nucleus and cytosol of paraffin-embedded mouse ovary tissue using a Casein Kinase 2 beta polyclonal antibody (Product # PA5-27416) at a dilution of 1:500.

CK2 beta Antibody in Western Blot (WB) — Western blot analysis of Casein Kinase 2 beta using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Casein Kinase 2 beta polyclonal antibody (Product # PA5-27416) at a dilution of 1:5000.

CK2 beta Antibody in ChIP assay (ChIP) — Chromatin immunoprecipitation analysis of Casein Kinase II beta was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a Casein Kinase II beta polyclonal antibody (Product # PA5-27416). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-Dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:5000

Published Applications

Immunohistochemistry (IHC)

See 1 publication below

Immunoprecipitation (IP)

See 1 publication below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 215 of Human Casein Kinase 2 beta

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 40% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544892

Product Specific Information

PA5-27416 targets Casein Kinase 2 beta in ChIP, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.

The PA5-27416 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 215 of Human Casein Kinase 2 beta.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alternative name: G5a; alternative name: G5a, phosvitin; casein kinase 2, beta polypeptide; casein kinase II b subunit splicing isoform 132; casein kinase II b subunit splicing isoform 247; casein kinase II b subunit splicing isoform 318; casein kinase II b subunit splicing isoform 660; casein kinase II b subunit splicing isoform 806; Casein kinase II beta subunit; Casein kinase II subunit beta; chimera CSNK2B-LY6G5B splicing isoform 1072; chimera CSNK2B-LY6G5B splicing isoform 1103; chimera CSNK2B-LY6G5B splicing isoform 532; chimera CSNK2B-LY6G5B splicing isoform 560; chimera CSNK2B-LY6G5B splicing isoform 562; CK II beta; CK2B; CK2N; CSK2B; CSNK2B-LY6G5B-1072; CSNK2B-LY6G5B-1103; CSNK2B-LY6G5B-532; CSNK2B-LY6G5B-560; CSNK2B-LY6G5B-562; G5A; Phosvitin; Protein G5a

Gene Aliases: CK II beta; CK2B; CK2N; CSK2B; CSNK2B; G5A

UniProt ID: (Human) P67870, (Mouse) P67871

Entrez Gene ID: (Human) 1460, (Mouse) 13001

Molecular Function: enzyme modulator kinase modulator