Invitrogen

CK2 beta Polyclonal Antibody

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CK2 beta Antibody in Immunocytochemistry (ICC/IF) — CK2 beta Polyclonal Antibody detects Casein Kinase 2 beta protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Casein Kinase 2 beta stained by CK2 beta Polyclonal Antibody (Product # PA5-27416) diluted at 1:500.

CK2 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — CK2 beta Polyclonal Antibody detects Casein Kinase 2 beta protein at nucleus and cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. CK2 beta Polyclonal Antibody (Product # PA5-27416) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

CK2 beta Antibody in Western Blot (WB) — Western blot analysis of CK2 beta was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CK2 beta Polyclonal Antibody (Product # PA5-27416) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

CK2 beta Antibody in ChIP assay (ChIP) — Chromatin immunoprecipitation analysis of Casein Kinase II beta was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a Casein Kinase II beta polyclonal antibody (Product # PA5-27416). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

CK2 beta Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 6. CK2 directly interacts with m18 and represses HDAC function. ( A ) Immunoprecipitation (IP) of m18 was performed in lysates of cells expressing m18-3xFlag or empty 3xFlag vector, and the products were analyzed for FLAG and CK2beta by western blot. Data are representative of three independent experiments. ( B ) Mouse fibroblasts were treated with CK2 inhibitor TBBt in conjunction with zVAD or zVAD alone and analyzed for RAE-1 expression by flow cytometry. Data is representative of three independent experiments. ( C ) Representative image of fibroblasts expressing m18-RFP and stained for HDAC3 pS424. ( D ) Quantification of HDAC3 pS424 levels in cells expressing m18-RFP or RFP control vector from compared to non-transfected controls in same field of view. Red bars are representative of mean+-SEM. Data are representative of three independent experiments. ****p<0.00005. n.s., not significant. (Student's T-test). DOI: http://dx.doi.org/ Figure 6--figure supplement 1. m18 expression does not change HDAC3 levels. ( A ) Representative picture of HDAC3 staining in m18-RFP transfected cells. ( B ) Nuclear staining of HDAC3 was quantified in FIJI. Data are representative of mean+-SEM. Data are representative of three independent experiments. n.s., Not significant (Students T-test). DOI: http://dx.doi.org/

Product Details

PA5-27416

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication
ChIP assay (ChIP)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 215 of Human Casein Kinase 2 beta View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.77 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544892

Product Specific Information

Recommended positive controls: MCF-7, NIH-3T3.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Depletion of Numb and Numblike in Murine Lung Epithelial Cells Ameliorates Bleomycin-Induced Lung Fibrosis by Inhibiting the β-Catenin Signaling Pathway.

Frontiers in cell and developmental biology

Ianni A,Hofmann M,Kumari P,Tarighi S,Al-Tamari HM,Görgens A,Giebel B,Nolte H,Krüger M,Salwig I,Pullamsetti SS,Günther A,Schneider A,Braun T

Published figure using CK2 beta polyclonal antibody (Product # PA5-27416) in Western Blot

Tue Jun 15 00:00:00 EDT 2021

A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression.

eLife

Greene TT,Tokuyama M,Knudsen GM,Kunz M,Lin J,Greninger AL,DeFilippis VR,DeRisi JL,Raulet DH,Coscoy L

PA527416 was used in immunohistochemistry to show that cytomegalovirus encoded protein m18 drives expression of the RAE-1 family of NKG2D ligands

Tue Nov 22 00:00:00 EST 2016

A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression.

eLife

Greene TT,Tokuyama M,Knudsen GM,Kunz M,Lin J,Greninger AL,DeFilippis VR,DeRisi JL,Raulet DH,Coscoy L

PA527416 was used in immunohistochemistry to show that cytomegalovirus encoded protein m18 drives expression of the RAE-1 family of NKG2D ligands

Tue Nov 22 00:00:00 EST 2016

A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression.

eLife

Greene TT,Tokuyama M,Knudsen GM,Kunz M,Lin J,Greninger AL,DeFilippis VR,DeRisi JL,Raulet DH,Coscoy L

PA527416 was used in immunohistochemistry to show that cytomegalovirus encoded protein m18 drives expression of the RAE-1 family of NKG2D ligands

Tue Nov 22 00:00:00 EST 2016

Bioinformatics

Protein Aliases: alternative name: G5a, phosvitin; casein kinase 2, beta polypeptide; casein kinase II b subunit splicing isoform 132; casein kinase II b subunit splicing isoform 247; casein kinase II b subunit splicing isoform 318; casein kinase II b subunit splicing isoform 660; casein kinase II b subunit splicing isoform 806; Casein kinase II beta subunit; Casein kinase II subunit beta; chimera CSNK2B-LY6G5B splicing isoform 1072; chimera CSNK2B-LY6G5B splicing isoform 1103; chimera CSNK2B-LY6G5B splicing isoform 532; chimera CSNK2B-LY6G5B splicing isoform 560; chimera CSNK2B-LY6G5B splicing isoform 562; CK II beta; CSNK2B-LY6G5B-1072; CSNK2B-LY6G5B-1103; CSNK2B-LY6G5B-532; CSNK2B-LY6G5B-560; CSNK2B-LY6G5B-562; DADB-127H9.2; MGC138222; MGC138224; Phosvitin; Protein G5a

Gene Aliases: CK II beta; CK2B; CK2N; CSK2B; CSNK2B; G5A

UniProt ID: (Human) P67870, (Mouse) P13862

Entrez Gene ID: (Human) 1460, (Mouse) 13001

Molecular Function: kinase modulator