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          • Primary Antibodies ›
          • SRC Antibodies

          Proteintech

          c-SRC Monoclonal Antibody (5E10C4), CoraLite® Plus 488

          View all (70) SRC antibodies

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          Cite c-SRC Monoclonal Antibody (5E10C4), CoraLite® Plus 488

          Additional Information:
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          • Antibody Testing Data (1)
          c-SRC Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          c-SRC Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          c-SRC Antibody (CL488-60315) in Flow

          1X10^6 MCF-7 cells were intracellularly stained with 0.4 µg CoraLite® Plus 488 Anti-Human c-SRC (CL488-60315, Clone:5E10C4) (red), or 0.4 µg Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          c-SRC Antibody in Flow Cytometry (Flow)
          c-SRC Monoclonal Antibody (5E10C4), CoraLite® Plus 488

          Product Details

          CL488-60315

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.4 µg/1x10^6 cell suspension
          -
          Product Specifications

          Species Reactivity

          Hamster, Human, Mouse

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          5E10C4

          Immunogen

          Recombinant protein
          View immunogen

          Conjugate

          CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488
          • Unconjugated
          • CoraLite 594
          • Request custom conjugation

          Excitation/Emission Max

          493/522 nm

          Form

          Liquid

          Purification

          Protein A

          Storage buffer

          PBS with 0.5% BSA, 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, store in dark

          Shipping conditions

          Wet ice

          Product Specific Information

          Immunogen sequence: MGSNKSKPKD ASQRRRSLEP AENVHGAGGG AFPASQTPSK PASADGHRGP SAAFAPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGVTTFV ALYDYESRTE TDLSFKKGER LQIVNNTEGD WWLAHSLSTG QTGYIPSNYV APSDSIQAEE WYFGKITRRE SERLLLNAEN PRGTFLVRES ETTKGAYCLS VSDFDNAKGL NVKHYKIRKL DSGGFYITSR TQFNSLQQLV AYYSKHADGL CHRLTTVCPT SKPQTQGLAK DAWEIPRESL RLEVKLGQGC FGEVWMGTWN GTTRVAIKTL KPGTMSPEAF LQEAQVMKKL RHEKLVQLYA VVSEEPIYIV TEYMSKGSLL DFLKGETGKY LRLPQLVDMA AQIASGMAYV ERMNYVHRDL RAANILVGEN LVCKVADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG RFTIKSDVWS FGILLTELTT KGRVPYPGMV NREVLDQVER GYRMPCPPEC PESLHDLMCQ CWRKEPEERP TFEYLQAFLE DYFTSTEPQY QPGENL

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; RP5-823N20.1; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; unnamed protein product; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

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          Gene Aliases: ASV; AW259666; c-SRC; p60-Src; pp60c-src; SRC; SRC1; THC6

          View more View less

          UniProt ID: (Human) P12931, (Mouse) P05480

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          Entrez Gene ID: (Human) 6714, (Chinese hamster) 100757188, (Mouse) 20779

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          Function(s)
          nucleotide binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding phospholipase activator activity kinase activity transferase activity enzyme binding protein domain specific binding heme binding protein tyrosine kinase activator activity receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding cadherin binding ephrin receptor binding ATPase binding phosphoprotein binding BMP receptor binding connexin binding scaffold protein binding protein kinase C binding insulin receptor binding protein C-terminus binding transferase activity, transferring phosphorus-containing groups protein kinase binding estrogen receptor binding protein complex binding cell adhesion molecule binding hormone receptor binding growth factor receptor binding
          Process(es)
          immune system process protein phosphorylation cell cycle cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway cell proliferation positive regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration phosphorylation cell migration peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation forebrain development positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of protein homooligomerization regulation of intracellular estrogen receptor signaling pathway adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis innate immune response bone resorption positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell adhesion negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway protein autophosphorylation neurotrophin TRK receptor signaling pathway oogenesis positive regulation of cytokine secretion progesterone receptor signaling pathway positive regulation of protein transport regulation of cell cycle negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly angiotensin-activated signaling pathway involved in heart process positive regulation of canonical Wnt signaling pathway positive regulation of intracellular signal transduction positive regulation of lamellipodium morphogenesis positive regulation of DNA biosynthetic process regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis negative regulation of transcription from RNA polymerase II promoter stimulatory C-type lectin receptor signaling pathway neutrophil activation involved in immune response negative regulation of inflammatory response to antigenic stimulus signal transduction signal complex assembly positive regulation of protein processing macroautophagy protein deubiquitination platelet activation T cell costimulation negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of telomere maintenance positive regulation of integrin activation toll-like receptor 3 signaling pathway regulation of toll-like receptor 3 signaling pathway positive regulation of dephosphorylation negative regulation of hippo signaling Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway angiotensin-activated signaling pathway TORC1 signaling vasodilation odontogenesis regulation of vascular permeability proteasome-mediated ubiquitin-dependent protein catabolic process regulation of bone resorption positive regulation of Notch signaling pathway positive regulation of glycolytic process viral entry into host cell vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway focal adhesion assembly leukocyte migration positive regulation of small GTPase mediated signal transduction intestinal epithelial cell development interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of protein serine/threonine kinase activity regulation of heart rate by cardiac conduction positive regulation of protein localization to nucleus negative regulation of neutrophil activation negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of platelet-derived growth factor receptor-beta signaling pathway
          It has to be done as per old AB suggested Products section.

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