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Proteintech
IL-12 Monoclonal Antibody (1E12H6), CoraLite® Plus 488
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FIGURE: 1 / 2
IL-12 Antibody (CL488-65277) in Flow
1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 µg CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone: 1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 µg CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone: 1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
CL488-65277
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1E12H6
Immunogen
IL-12 fusion protein HZ-1256
Conjugate
CoraLite® Plus 488
CoraLite® Plus 488
CoraLite® Plus 488
Excitation/Emission Max
493/522 nm
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
Target Information
Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12, subunit p35; IL12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; Interleukin12; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF/CLMF; IL12p35; 35kD
Gene Aliases: CLMF; IL-12A; NFSK; NKSF1; P35
UniProt ID: (Human) P29459
Entrez Gene ID: (Human) 3592
positive regulation of T cell mediated cytotoxicity
negative regulation of T-helper 1 type immune response
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
immune response
response to virus
response to UV-B
cell migration
regulation of B cell proliferation
response to lipopolysaccharide
negative regulation of interleukin-17 production
positive regulation of interferon-gamma production
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
positive regulation of mononuclear cell proliferation
positive regulation of smooth muscle cell apoptotic process
interleukin-12-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of cell adhesion
positive regulation of natural killer cell mediated cytotoxicity
positive regulation of JAK-STAT cascade
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
negative regulation of protein secretion
defense response to Gram-positive bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
interleukin-35-mediated signaling pathway
extrinsic apoptotic signaling pathway
cellular response to virus
negative regulation of vascular endothelial growth factor signaling pathway
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
positive regulation of dendritic cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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