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          • Primary Antibodies ›
          • AMPK alpha-1 Antibodies

          Proteintech

          AMPK alpha Monoclonal Antibody (1H8E1), CoraLite® Plus 488

          1 Reference
          View all (70) AMPK alpha-1 antibodies

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          Cite AMPK alpha Monoclonal Antibody (1H8E1), CoraLite® Plus 488

          Additional Information:
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          • Antibody Testing Data (2)
          AMPK alpha Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
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          AMPK alpha Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          AMPK alpha Antibody (CL488-66536) in IHC (PFA)

          Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100 {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          AMPK alpha Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          AMPK alpha Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

          Product Details

          CL488-66536

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1H8E1

          Immunogen

          Recombinant Protein

          Conjugate

          CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488
          • Unconjugated
          • Request custom conjugation

          Excitation/Emission Max

          493/522 nm

          Form

          Liquid

          Concentration

          2 mg/mL

          Amount

          200 µg

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.3, with 0.5% BSA, 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, store in dark

          Shipping conditions

          Wet ice

          Target Information

          Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; activated protein kinase; AMP -activate kinase alpha 1 subunit; AMP activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha-1 chain; AMPK subunit alpha-1; AMPK subunit pAMPK; AMPK, alpha, 1; AMPK-alpha-2 chain; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; MGC33776; MGC57364; pAMPK; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 1 catalytic subunit; Tau-protein kinase PRKAA1; unnamed protein product

          View more View less

          Gene Aliases: AI194361; AI450832; AL024255; AMPK; AMPK alpha 1; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1

          View more View less

          UniProt ID: (Human) Q13131, (Rat) P54645, (Mouse) Q5EG47

          View more View less

          Entrez Gene ID: (Human) 5562, (Rat) 65248, (Mouse) 105787

          View more View less

          Function(s)
          nucleotide binding chromatin binding protein kinase activity protein serine/threonine kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding kinase activity transferase activity macromolecular complex binding metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau protein binding tau-protein kinase activity protein serine kinase activity histone H2BS36 kinase activity protein C-terminus binding kinase binding histone serine kinase activity eukaryotic elongation factor-2 kinase activator activity [acetyl-CoA carboxylase] kinase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
          Process(es)
          autophagosome assembly response to hypoxia cytoplasmic translation negative regulation of T cell mediated immune response to tumor cell positive regulation of T cell mediated immune response to tumor cell glucose metabolic process chromatin organization chromatin remodeling lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process phosphatidylethanolamine biosynthetic process phosphatidylcholine biosynthetic process steroid biosynthetic process cholesterol biosynthetic process autophagy lysosome organization signal transduction steroid metabolic process cholesterol metabolic process positive regulation of cell proliferation lipid biosynthetic process cellular response to starvation response to xenobiotic stimulus response to UV cold acclimation response to gamma radiation positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression response to activity Wnt signaling pathway sterol biosynthetic process fatty acid oxidation response to caffeine SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization cellular response to nutrient levels negative regulation of TOR signaling cellular response to stress cellular response to amino acid starvation cellular response to oxidative stress TORC1 signaling cellular response to glucose starvation response to hydrogen peroxide glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process regulation of vascular permeability proteasome-mediated ubiquitin-dependent protein catabolic process response to estrogen positive regulation of cholesterol biosynthetic process positive regulation of glycolytic process positive regulation of transcription, DNA-templated negative regulation of translational initiation positive regulation of translational initiation negative regulation of glucosylceramide biosynthetic process negative regulation of insulin receptor signaling pathway rhythmic process positive regulation of skeletal muscle tissue development protein stabilization negative regulation of T cell activation positive regulation of T cell activation negative regulation of lipid catabolic process fatty acid homeostasis regulation of vesicle-mediated transport protein localization to lysosome motor behavior CAMKK-AMPK signaling cascade regulation of stress granule assembly protein-containing complex assembly neuron cellular homeostasis cellular response to hydrogen peroxide regulation of microtubule cytoskeleton organization cellular response to calcium ion cellular response to glucose stimulus cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia cellular response to xenobiotic stimulus protein K6-linked ubiquitination positive regulation of release of cytochrome c from mitochondria energy homeostasis hepatocyte apoptotic process negative regulation of protein localization to nucleus positive regulation of transcription from mitochondrial promoter positive regulation of cellular protein localization negative regulation of hepatocyte apoptotic process positive regulation of protein targeting to mitochondrion positive regulation of adipose tissue development negative regulation of TORC1 signaling positive regulation of TORC1 signaling negative regulation of tubulin deacetylation lipid droplet disassembly protein localization to lipid particle activation of MAPK activity transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation negative regulation of translation histone-serine phosphorylation positive regulation of gluconeogenesis positive regulation of fatty acid oxidation positive regulation of glucose import protein heterooligomerization cellular response to organonitrogen compound response to camptothecin regulation of energy homeostasis negative regulation of glucose import in response to insulin stimulus phosphorylation chromatin modification regulation of peptidyl-serine phosphorylation intracellular signal transduction
          It has to be done as per old AB suggested Products section.

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