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FAK Monoclonal Antibody (2C5B9), CoraLite® Plus 488

View all (108) FAK antibodies

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Product Details

CL48866258100UL

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:50-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2c

Class

Monoclonal

Type

Antibody

Clone

2C5B9

Conjugate

CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488

Excitation/Emission Max

493/522 nm

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.5% BSA

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; unnamed protein product

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Gene Aliases: FADK; FADK 1; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346

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Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614

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Function(s)
nucleotide binding actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding ATP binding JUN kinase binding kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity signal transducer activity receptor binding transferase activity, transferring phosphorus-containing groups protein complex binding identical protein binding phosphatidylinositol 3-kinase binding
Process(es)
MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development regulation of cell-matrix adhesion heart morphogenesis axon guidance regulation of endothelial cell migration positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition positive regulation of macrophage chemotaxis positive regulation of fibroblast migration cell migration establishment of cell polarity cell differentiation detection of muscle stretch netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity regulation of cell differentiation vascular endothelial growth factor receptor signaling pathway cell motility regulation of multicellular organismal process regulation of cytoskeleton organization positive regulation of wound healing vascular endothelial cell response to oscillatory fluid shear stress positive regulation of macrophage proliferation cytoskeleton organization response to mechanical stimulus response to glucose response to organic substance response to organonitrogen compound response to organic cyclic compound response to estradiol response to drug fat cell differentiation response to arsenic-containing substance
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