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Phospho-MST1/MST2 (Thr183, Thr180) Recombinant Rabbit Monoclonal Antibody (1P6), CoraLite® Plus 488
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FIGURE: 1 / 1
Phospho-MST1/MST2 (Thr183, Thr180) Antibody (CL48880093100UL) in Flow
1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 µg CoraLite 488 Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (Product # CL48880093100UL), Clone:1P6), or 0.5 µg Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
Product Details
CL48880093100UL
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
proprietary
Class
Recombinant Monoclonal
Type
Antibody
Clone
1P6
Conjugate
Excitation/Emission Max
493/522 nm
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
Target Information
STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; hippo homolog; KB-1458E12.1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; Mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-1; MST-2; MST1/C STK4; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STE20-like kinase MST2; unnamed protein product
Gene Aliases: KRS1; KRS2; MST1; MST2; STK3; STK4; YSK3
UniProt ID: (Human) Q13188, (Human) Q13043
Entrez Gene ID: (Human) 6788, (Human) 6789
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
identical protein binding
protein serine/threonine kinase activator activity
metal ion binding
protein serine kinase activity
transcription regulator activator activity
protein homodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
cell morphogenesis
patterning of blood vessels
neural tube formation
positive regulation of protein phosphorylation
endocardium development
protein phosphorylation
protein import into nucleus
apoptotic process
signal transduction
central nervous system development
cell proliferation
negative regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
anatomical structure morphogenesis
peptidyl-serine phosphorylation
keratinocyte differentiation
positive regulation of peptidyl-serine phosphorylation
organ growth
hippo signaling
positive regulation of hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
regulation of MAPK cascade
positive regulation of fat cell differentiation
negative regulation of organ growth
protein autophosphorylation
epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein stabilization
protein tetramerization
canonical Wnt signaling pathway
primitive hemopoiesis
cell differentiation involved in embryonic placenta development
regulation of cell differentiation involved in embryonic placenta development
negative regulation of canonical Wnt signaling pathway
hepatocyte apoptotic process
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of hepatocyte apoptotic process
positive regulation of substrate-dependent cell migration, cell attachment to substrate
JNK cascade
regulation of gene expression
protein kinase B signaling
positive regulation of JNK cascade
centrosome separation
positive regulation of protein kinase B signaling
protein localization to centrosome
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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