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Proteintech
PCK1 Monoclonal Antibody (2F11B5), CoraLite® 555
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Product Details
CL555-66862
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F11B5
Immunogen
Recombinant protein
Conjugate
Excitation/Emission Max
555/570 nm
Form
Liquid
Purification
Protein G
Storage buffer
PBS with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: EGEKKYLAAA FPSACGKTNL AMMNPSLPGW KVECVGDDIA WMKFDAQGHL RAINPENGFF GVAPGTSVKT NPNAIKTIQK NTIFTNVAET SDGGVYWEGI DEPLASGVTI TSWKNKEWSS EDGEPCAHPN SRFCTPASQC PIIDAAWESP EGVPIEGIIF GGRRPAGVPL VYEALSWQHG VFVGAAMRSE ATAAAEHKGK IIMHDPFAMR PFFGYNFGKY LAHWLSMAQH PAAKLPKIFH VNWFRKDKEG KFLWPGFGEN SRVLEWMFNR IDGKASTKLT PIGYIPKEDA LNLKGLGHIN MMELFSISKE FWEKEVEDIE KYLEDQVNAD LPCEIEREIL ALKQRISQM
Target Information
Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:8724; MGC22652; PE PEPCKC; PEP carboxykinase; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; Serine-protein kinase PCK1
Gene Aliases: AI265463; GTP; PCK; Pck-1; PCK1; PEPCK; PEPCK-C; PEPCK1; PEPCKC; RATPEPCK
UniProt ID: (Pig) A0A4X1UM84, (Human) P35558, (Rat) P07379, (Mouse) Q9Z2V4
Entrez Gene ID: (Pig) 100144531, (Human) 5105, (Rat) 362282, (Mouse) 18534
glucose metabolic process
gluconeogenesis
oxaloacetate metabolic process
internal protein amino acid acetylation
response to activity
response to insulin
glucose homeostasis
glycerol biosynthetic process from pyruvate
cellular response to potassium ion starvation
positive regulation of transcription from RNA polymerase II promoter in response to acidic pH
lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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