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Proteintech

Caspase 8/p43/p18 Monoclonal Antibody (2B9H8), CoraLite® 594

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Product Details

CL594-66093

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:50-1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2B9H8

Immunogen

Fusion Protein

Conjugate

CoraLite® 594 CoraLite® 594 CoraLite® 594

Excitation/Emission Max

593/614 nm

Form

Liquid

Concentration

2 mg/mL

Amount

200 µg

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 0.5% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, store in dark

Shipping conditions

Wet ice

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8 precursor; caspase-8s; Caspase8; EC 3.4.22.61; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLJ17672; ICE-like apoptotic protease 5; ICE8; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581

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Gene Aliases: ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5

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UniProt ID: (Human) Q14790

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Entrez Gene ID: (Human) 841

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Function(s)
endopeptidase activity cysteine-type endopeptidase activity death receptor binding protein binding peptidase activity cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process ubiquitin protein ligase binding death effector domain binding identical protein binding macromolecular complex binding scaffold protein binding
Process(es)
angiogenesis regulation of cytokine production regulation of immune system process proteolysis apoptotic process heart development extrinsic apoptotic signaling pathway via death domain receptors positive regulation of signal transduction regulation of tumor necrosis factor-mediated signaling pathway protein processing natural killer cell activation cell differentiation protein catabolic process macrophage differentiation positive regulation of cell migration response to cobalt ion response to estradiol response to lipopolysaccharide positive regulation of interleukin-1 beta production response to tumor necrosis factor TRAIL-activated apoptotic signaling pathway T cell activation B cell activation regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process regulation of innate immune response response to ethanol positive regulation of macrophage differentiation positive regulation of proteolysis animal organ development protein maturation negative regulation of necroptotic process syncytiotrophoblast cell differentiation involved in labyrinthine layer development pyroptosis cellular response to mechanical stimulus death-inducing signaling complex assembly response to anesthetic apoptotic signaling pathway extrinsic apoptotic signaling pathway execution phase of apoptosis self proteolysis positive regulation of execution phase of apoptosis
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