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Proteintech
NEDD4 Recombinant Rabbit Monoclonal Antibody (230478F1), CoraLite® Plus 750
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Product Details
CL750-83112
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
230478F1
Immunogen
NEDD4 fusion protein Ag16291
Conjugate
Excitation/Emission Max
755/780 nm
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: TVLEDSYRRI MGVKRADFLK ARLWIEFDGE KGLDYGGVAR EWFFLISKEM FNPYYGLFEY SATDNYTLQI NPNSGLCNED HLSYFKFIGR VAGMAVYHGK LLDGFFIRPF YKMMLHKPIT LHDMESVDSE YYNSLRWILE NDPTELDLRF IIDEELFGQT HQHELKNGGS EIVVTNKNKK EYIYLVIQWR FVNRIQKQMA AFKEGFFELI PQDLIKIFDE NELELLMCGL GDVDVNDWRE HTKYKNGYSA NHQVIQWFWK AVLMMDSEKR IRLLQFVTGT SRVPMNGFAE LYGSNGPQSF TVEQWGTPEK LPRAHTCFNR LDLPPYESFE ELWDKLQMAI ENTQGFDGVD
Target Information
The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; KIAA0093; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; PIG53; receptor-potentiating factor 1; unnamed protein product
Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; KIAA0093; mKIAA0093; Nedd-4; NEDD4; NEDD4-1; Nedd4a; PIG53; RPF1
UniProt ID: (Human) P46934, (Rat) Q62940, (Mouse) P46935
Entrez Gene ID: (Human) 4734, (Rat) 25489, (Mouse) 17999
ubiquitin-protein transferase activity
protein binding
channel inhibitor activity
transferase activity
potassium channel inhibitor activity
sodium channel inhibitor activity
enzyme binding
protein domain specific binding
beta-2 adrenergic receptor binding
ionotropic glutamate receptor binding
ubiquitin binding
ion channel binding
phosphoserine binding
phosphothreonine binding
ubiquitin protein ligase activity
RNA polymerase binding
proline-rich region binding
protein C-terminus binding
ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
adaptive immune response
outflow tract morphogenesis
endocardial cushion development
transcription from RNA polymerase II promoter
ubiquitin-dependent protein catabolic process
protein monoubiquitination
protein targeting to lysosome
sodium ion transport
immune response
cellular response to DNA damage stimulus
lysosomal transport
nervous system development
neuromuscular junction development
regulation of cell communication
negative regulation of sodium ion transport
regulation of macroautophagy
protein ubiquitination
regulation of signaling
negative regulation of vascular endothelial growth factor receptor signaling pathway
neuron projection development
receptor internalization
receptor catabolic process
cellular response to UV
T cell activation
regulation of protein catabolic process
regulation of membrane potential
glucocorticoid receptor signaling pathway
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
development involved in symbiotic interaction
innate immune response
positive regulation of protein catabolic process
viral budding
positive regulation of nucleocytoplasmic transport
blood vessel morphogenesis
regulation of dendrite morphogenesis
regulation of synapse organization
progesterone receptor signaling pathway
response to calcium ion
establishment of localization in cell
positive regulation of protein kinase B signaling
regulation of biological quality
protein K63-linked ubiquitination
negative regulation of potassium ion export across plasma membrane
negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
positive regulation of phosphatidylinositol 3-kinase signaling
response to denervation involved in regulation of muscle adaptation
transmission of virus
regulation of ion transmembrane transport
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
regulation of potassium ion transmembrane transporter activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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