|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: N(171) H G F L S A D Q QL I K(183)|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4306 detects CLAC-P (NC2-1 region) in human and mouse samples.
PA1-4306 has been successfully used in Western blot procedures. By Western blot this antibody detects bands of 50, 70, and 100 kDa representing CLAC-P in formic acid extracts of Alzheimer and quote;s disease brain.
PA1-4306 immunizing peptide corresponds to amino acid residues 171-183 of the NC2-1 region of human and mouse CLAC-P.
Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.