|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: D(430) Y N G N L H E A L Q R I T(443) of the NC3 region of human and murine CLAC-P|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1:100 - 1:200|
|Western Blot (WB)||1:1000 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4308 detects CLAC-P (NC3 region) in human and mouse samples.
PA1-4308 has been successfully used in Western blotting and immunohistochemistry procedures. By Western blot, this antibody detects a ~70 kDa protein representing CLAC-P (NC3 region) in stable HEK293T cell lines expressing CLAC-P (NC3 region). PA1-4308 does not cross react with COLXIII or COLXXIII proteins.
PA1-4308 immunizing peptide corresponds to amino acid residues 430-443 of the NC3 region of human and mouse CLAC-P.
Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid freeze/thaw cycles.
Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.