Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CLIF Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
CLIF Antibody (PA5-114384) in WB
Western blot analysis of CLIF in the following samples: Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat ovary tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse testis tissue lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash... View More
Product Details
PA5-114384
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ARNTL2 recombinant protein (Position: E461-T575).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, human A549 whole cell, human HepG2 whole cell, rat ovary tissue, mouse lung tissue, mouse testis tissue. Flow: A431 cell, HL-60 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER, ARNT, SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein, the structurally related MOP4, and hypoxia-inducible factors, such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: aryl hydrocarbon receptor nuclear translocator-like 2; Aryl hydrocarbon receptor nuclear translocator-like protein 2; Basic helix-loop-helix ARNT-like protein 2; Basic-helix-loop-helix-PAS protein MOP9; bHLHe6; Brain and muscle ARNT-like 2; brain and muscle Arnt-like protein 2 variant d; brain-muscle-ARNT-like protein 2; Class E basic helix-loop-helix protein 6; CLIF; CYCLE-like factor; Member of PAS protein 9; MGC149671; MGC149672; PAS domain-containing protein 9; transcription factor BMAL2
Gene Aliases: 4632430A05Rik; Arnt4; ARNTL2; BHLHE6; BMAL2; CLIF; MOP9; PASD9
UniProt ID: (Human) Q8WYA1, (Mouse) Q2VPD4
Entrez Gene ID: (Human) 56938, (Mouse) 272322, (Rat) 362464
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein dimerization activity
E-box binding
DNA binding
signal transducer activity
protein binding
molecular_function
basic helix-loop-helix transcription factor
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
circadian rhythm
entrainment of circadian clock
positive regulation of circadian rhythm
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
signal transduction
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support