Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:200-1:2,000 | View 3 publications 3 publications |
Immunohistochemistry (IHC) |
- | View 4 publications 4 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
1:100-1:1,000 | - |
Immunohistochemistry - Free Floating (IHC (Free)) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:10-1:200 | View 1 publication 1 publication |
Gel Shift (GS) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Hamster, Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic Peptide: S(11) S I V D R D D S S I F D G L V E E D D(30) C |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2082586 |
PA1-520 detects CLOCK from human, mouse, rat and hamster tissues.
PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus.
PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. # PEP-072) is available for use in neutralization and control experiments.
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHe8; circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock circadian regulator; clock homolog; hCLOCK; mCLOCK; rCLOCK
Gene Aliases: 5330400M04Rik; BHLHE8; CLOCK; I79_020252; KAT13D; KIAA0334
UniProt ID: (Human) O15516, (Mouse) O08785, (Rat) Q9WVS9
Entrez Gene ID: (Human) 9575, (Chinese hamster) 100770092, (Mouse) 12753, (Rat) 60447
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support