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      CLOCK Polyclonal Antibody

      See 5 other CLOCK antibodies ›
      CLOCK Polyclonal Antibody for Western Blot, IF, ICC, IHC (F) and IHC (P)

      Catalog#: PA1-520 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA1-520 200 µL
      375.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Hamster, Human, Mouse, Rat
      Published species reactivity Mouse, Human, Not Applicable
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Synthetic Peptide: S(11) S I V D R D D S S I F D G L V E E D D(30) C
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer PBS with 1mg/ml BSA
      Contains 0.05% sodium azide
      Storage Conditions -20° C, Avoid Freeze/Thaw Cycles
      Tested Applications Dilution *
      Immunocytochemistry (ICC) 1:10-1:200
      Immunofluorescence (IF) 1:10-1:200
      Immunohistochemistry (Frozen) (IHC (F)) 1:100-1:1000
      Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
      Western Blot (WB) 1:200

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Immunohistochemistry - Free Floating (IHC (Free)) See 1 publications below
      Gel Shift (GS) See 1 publications below
      Western Blot (WB) See 1 publications below
      Immunohistochemistry (IHC) See 3 publications below

      Product Specific Information

      PA1-520 detects CLOCK from human, mouse, rat and hamster tissues.

      PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus.

      PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. # PEP-072) is available for use in neutralization and control experiments.

      Background/Target Information

      Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Immunohistochemistry - Free Floating

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse 1:500 Diurnal oscillation of amygdala clock gene expression and loss of synchrony in a mouse model of depression.
      PA1-520 was used in immunohistochemistry - free floating to discuss the contribution of the circadian system to depression.       		Savalli G,Diao W,Schulz S,Todtova K,Pollak DD The international journal of neuropsychopharmacology (18:null) 2014

      Gel Shift

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable Not Cited p53 regulates Period2 expression and the circadian clock.
      PA1-520 was used in EMSA to assess the role of p53 in regulating Period2 expression and the circadian clock       		Miki T,Matsumoto T,Zhao Z,Lee CC Nature communications (4:null) 2013

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse 1:250 Circadian rhythms regulate amelogenesis.
      PA1-520 was used in western blot to study the circadian regulation of amelogenesis       		Zheng L,Seon YJ,Mourão MA,Schnell S,Kim D,Harada H,Papagerakis S,Papagerakis P Bone (55:158) 2013

      Immunohistochemistry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse 1:500 Expression of clock proteins in developing tooth.
      PA1-520 was used in immunohistochemistry to study the role of clock genes in developing tooth       		Zheng L,Papagerakis S,Schnell SD,Hoogerwerf WA,Papagerakis P Gene expression patterns : GEP (11:202) 2011
      Human 1:100 The E2F-regulated gene Chk1 is highly expressed in triple-negative estrogen receptor /progesterone receptor /HER-2 breast carcinomas.
      PA1-520 was used in immunohistochemistry to study the expression of Chk1 in triple-negative estrogen receptor/progesterone receptor/HER-2 breast carcinomas       		Verlinden L,Vanden Bempt I,Eelen G,Drijkoningen M,Verlinden I,Marchal K,De Wolf-Peeters C,Christiaens MR,Michiels L,Bouillon R,Verstuyf A Cancer research (67:6574) 2007
      Mouse 1:1,000 Non-cyclic and developmental stage-specific expression of circadian clock proteins during murine spermatogenesis.
      PA1-520 was used in immunohistochemistry to investigate the expression of circadian clock proteins during murine spermatogenesis       		Alvarez JD,Chen D,Storer E,Sehgal A Biology of reproduction (69:81) 2003

      Bioinformatics

      Protein Aliases:

      bHLHe8; circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Circadium Locomotor Output Cycles Kaput; Class E basic helix-loop-helix protein 8; clock homolog; hCLOCK; mCLOCK; rCLOCK

      Gene Aliases:

      5330400M04Rik; BHLHE8; CLOCK; KAT13D; KIAA0334

      UniProt ID:

      (Human) O15516, (Mouse) O08785, (Rat) Q9WVS9

      Entrez Gene ID:

      (Human) 9575, (Mouse) 12753, (Rat) 60447

      Molecular Function:

      basic helix-loop-helix transcription factor transcription factor

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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