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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C S(394) L P E T A A D K S Q D S G S D N (410) of mouse CLOCK.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-521 detects CLOCK from cells transfected with the mouse CLOCK gene.
PA1-521 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from cells transfected with the mouse CLOCK gene.
The PA1-521 immunogen is a synthetic peptide corresponding to residues C S(394) L P E T A A D K S Q D S G S D N (410) of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-521 immunizing peptide (Cat. # PEP-073) is available for use in neutralization and control experiments.
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||1:200 to 1:1,000||
Expression of circadian rhythm genes in gonadotropin-releasing hormone-secreting GT1-7 neurons.
PA1-521 was used in western blot to investigate the role of the clock machinery in GnRH neuronal function.
|Gillespie JM,Chan BP,Roy D,Cai F,Belsham DD||Endocrinology (144:5285)||2003|
circadian locomoter output cycles protein kaput; Circadium Locomotor Output Cycles Kaput
5330400M04Rik; Clock; KAT13D