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Invitrogen
CLOCK Polyclonal Antibody
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FIGURE: 1 / 3
CLOCK Antibody (PA3-16514) in WB
Western blot was performed using Anti-CLOCK Polyclonal Antibody (Product # PA3-16514) and a 95kDa band corresponding to CLOCK was observed across the cell lines and tissue extracts tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), MCF-7 (Lane 3), MCF-7 treated with Beta Estradiol (1uM for 24hrs) (Lane 4), T-47D (Lane 5), PC-3 (Lane 6), NIH/3T3 (Lane 7), tissue extracts of Mouse Testis (Lane 8), Mouse Skeletal muscle (Lane 9), Rat Skeletal muscle (Lane 10) and Mouse Thymus (Lane 11) were electrop... View More
Product Details
PA3-16514
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Two peptides derived from mouse CLOCK conjugated to albumin.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody has been tested against bacterially expressed fragment of CLOCK expressed in BL21 cells or the full length protein expressed in an in vitro translation system (reticulocyte lysate).
Target Information
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHe8; circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock homolog; hCLOCK; mCLOCK; rCLOCK
Gene Aliases: 5330400M04Rik; BHLHE8; CLOCK; KAT13D; KIAA0334
UniProt ID: (Human) O15516, (Mouse) O08785, (Rat) Q9WVS9
Entrez Gene ID: (Human) 9575, (Mouse) 12753, (Rat) 60447
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
signal transducer activity
transcription factor binding
transferase activity
transferase activity, transferring acyl groups
DNA damage checkpoint
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
signal transduction
spermatogenesis
circadian rhythm
histone acetylation
circadian regulation of gene expression
regulation of hair cycle
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
positive regulation of inflammatory response
regulation of insulin secretion
positive regulation of NF-kappaB transcription factor activity
response to redox state
cellular response to ionizing radiation
regulation of type B pancreatic cell development
negative regulation of glucocorticoid receptor signaling pathway
regulation of transcription from RNA polymerase II promoter
photoperiodism
entrainment of circadian clock
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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