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          • Primary Antibodies ›
          • CLPB Antibodies

          Invitrogen

          CLPB Polyclonal Antibody

          View all (8) CLPB antibodies

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          Cite CLPB Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          CLPB Antibody in Immunocytochemistry (ICC/IF)
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          CLPB Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          CLPB Antibody (PA5-143890) in ICC/IF

          Immunocytochemistry analysis of CLPB in A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CLPB Polyclonal antibody (Product # PA5-143890) using a dilution of 4 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CLPB Antibody in Immunocytochemistry (ICC/IF)
          CLPB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CLPB Antibody in Western Blot (WB)
          CLPB Antibody in Flow Cytometry (Flow)
          CLPB Polyclonal Antibody

          Product Details

          PA5-143890

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          0.5-1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          4 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human CLPB recombinant protein (Position: Q438-I707).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.01mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075104

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human HEK293 whole cell, huamn SW620 whole cell, huamn 22RV1 whole cell, huamn THP-1 whole cell, human SGC-7901 whole cell, monkey COS-7 whole cell, rat kidney tissue, mouse heart tissue, mouse HEPA1-6 whole cell. IHC: human mammary cancer tissue, human rectal cancer tissue. ICC/IF: A549 cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          CLPB may function as a regulatory ATPase and be related to secretion/protein trafficking process.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ankyrin-repeat containing bacterial clp fusion; caseinolytic mitochondrial matrix peptidase chaperone subunit B; caseinolytic peptidase B protein homolog; ClpB caseinolytic peptidase B homolog; ClpB homolog, mitochondrial AAA ATPase chaperonin; Mitochondrial disaggregase; suppressor of K+ transport defect 3; Suppressor of potassium transport defect 3; testicular secretory protein Li 11; unnamed protein product

          View more View less

          Gene Aliases: AL118244; ANKCLB; ANKCLP; CLPB; HSP78; MEGCANN; MGCA7; MGCA7A; SCN9; SKD3

          View more View less

          UniProt ID: (Human) Q9H078, (Mouse) Q60649, (Rat) Q9WTT2

          View more View less

          Entrez Gene ID: (Human) 81570, (Mouse) 20480, (Rat) 65041

          View more View less

          Function(s)
          nucleotide binding protein binding ATP binding hydrolase activity ATPase activity ATP-dependent protein disaggregase activity molecular_function protease
          Process(es)
          granulocyte differentiation cellular response to heat RIG-I signaling pathway antiviral innate immune response biological_process
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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