Invitrogen

CMKLR1 Polyclonal Antibody

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CMKLR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CMKLR1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CMKLR1 polyclonal antibody (Product # PA5-99441) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

CMKLR1 Antibody in Western Blot (WB) — Western blot analysis of CMKLR1 in hybridoma cell lysate (left lane: treated with blocking peptide). Samples were incubated with CMKLR1 polyclonal antibody (Product # PA5-99441).

Product Details

PA5-99441

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human CMKLR1(Accession Q99788), corresponding to amino acid residues T242-V263. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2818374

Target Information

Chemokine-like receptor 1 (CMKLR1) is a receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. CMKLR1 positively regulates adipogenesis and adipocyte metabolism and acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: chemerin receptor; chemokine receptor-like 1; Chemokine-like receptor 1; G-protein coupled chemoattractant-like receptor; G-protein coupled receptor 27; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor, Dez; resolvin E1 receptor

Gene Aliases: CHEMERINR; CHEMR23; CMKLR1; DEZ; Gpcr27; mcmklr1; RVER1

UniProt ID: (Human) Q99788, (Mouse) P97468

Entrez Gene ID: (Human) 1240, (Mouse) 14747, (Rat) 60669

Molecular Function: G-protein coupled receptor