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Invitrogen
CNOT1 Polyclonal Antibody
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FIGURE: 1 / 2
CNOT1 Antibody (PA5-119953) in WB
Western blot analysis of CNOT1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. CNOT1 Polyclonal Antibody (Product # PA5-119953) at 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Product Details
PA5-119953
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human CNOT1 aa 1-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Rat brain tissue lysates, SiHa.
Subcellular Location: Cytoplasm, Nucleus.
Target Information
Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenal gland protein AD-005; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 1; Negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1H; unnamed protein product
Gene Aliases: 6030411K04Rik; AA815922; AD-005; CDC39; CNOT1; D830048B13; HPE12; KIAA1007; NOT1; NOT1H; RGD1308009; VIBOS
UniProt ID: (Human) A5YKK6, (Mouse) Q6ZQ08
Entrez Gene ID: (Human) 23019, (Rat) 291841, (Mouse) 234594
RNA binding
poly(A)-specific ribonuclease activity
protein binding
protein domain specific binding
estrogen receptor binding
retinoic acid receptor binding
binding, bridging
armadillo repeat domain binding
molecular_function
protein complex scaffold
poly(A) RNA binding
mRNA polyadenylation factor
RNA metabolism protein
RNA processing factor
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
nuclear-transcribed mRNA poly(A) tail shortening
trophectodermal cell differentiation
regulation of translation
positive regulation of cytoplasmic mRNA processing body assembly
negative regulation of translation
gene silencing by RNA
negative regulation of intracellular estrogen receptor signaling pathway
gene silencing by miRNA
negative regulation of retinoic acid receptor signaling pathway
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of mRNA catabolic process
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
regulation of stem cell population maintenance
biological_process
RNA phosphodiester bond hydrolysis, exonucleolytic
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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