Search
Invitrogen
CNOT7 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
CNOT7 Antibody (PA5-101270) in ICC/IF
Immunocytochemistry analysis of CNOT7 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CNOT7 polyclonal antibody (Product # PA5-101270) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101270
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CNOT7(Accession Q9UIV1), corresponding to amino acid residues E243-S285.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CNOT7.
Target Information
CNOT7 (CCR4-NOT transcription complex subunit 7) has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. CNOT7 is a catalytic component of the CCR4-NOT complex, which is one of the major cellular mRNA deadenylases, and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. During miRNA-mediated repression, the complex seems to act as a transcriptional repressor during translational initiation, as well. Additional complex functions may be a consequence of its influence on mRNA expression. CNOT7 associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: BTG1-binding factor 1; CAF-1; Caf1a; carbon catabolite repressor protein (CCR4)-associative factor 1; catabolite repressor protein (CCR4)-associative factor 1; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 7; POP2 homolog; unnamed protein product
Gene Aliases: AU022737; CAF-1; CAF1; Caf1a; CNOT7; hCAF-1; Pop2
UniProt ID: (Human) Q9UIV1, (Mouse) Q60809
Entrez Gene ID: (Human) 29883, (Mouse) 18983
3'-5'-exoribonuclease activity
nucleic acid binding
transcription corepressor activity
RNA binding
nuclease activity
exonuclease activity
exoribonuclease activity
poly(A)-specific ribonuclease activity
protein binding
hydrolase activity
piRNA binding
metal ion binding
DNA-binding transcription factor binding
mRNA polyadenylation factor
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
nuclear-transcribed mRNA poly(A) tail shortening
deadenylation-dependent decapping of nuclear-transcribed mRNA
regulation of translation
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of gene expression
gene silencing by RNA
cytoplasmic mRNA processing body assembly
mRNA cleavage involved in gene silencing by miRNA
regulation of tyrosine phosphorylation of STAT protein
positive regulation of viral genome replication
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
negative regulation of type I interferon-mediated signaling pathway
positive regulation of mRNA catabolic process
piRNA-mediated gene silencing by mRNA destabilization
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
transcription, DNA-templated
regulation of transcription, DNA-templated
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
RNA phosphodiester bond hydrolysis, exonucleolytic
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support