Invitrogen

CNR2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

16 References

View all (15) CNR2 antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

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FIGURE: 1 / 8

CNR2 Antibody (PA1-744) in WB

Western blot analysis of Cannabinoid Receptor II was performed by loading 25 µg of PC-3 (lane 1) and Jurkat (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Cannabinoid Receptor II polyclonal antibody (Product # PA1-744) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Pl... View More

CNR2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CNR2 Antibody — Fig. 8 CB1 and CB2 expression, represented as median fluorescence intensity (left panel) in PBMC from 12 healthy controls and 12 MS patients under the specified stimulation conditions. Data are shown for lymphocyte/monocyte-gated cells. The right panel is a representative histogram of CB 1 and CB 2 expression in an MS patient's PBMCs in different stimulation conditions. Colour legend, Black: Isotype Control; Red: Unstimulated; Orange: +IL-1beta; Blue: +IL-6; Pink: +TNF-alpha. Results show a statistically significant increase in CB 2 expression between controls and MS patients after stimulation with IL-1beta, IL-6, and TNF-alpha. {TM}

Product Details

PA1-744

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:2500

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

1:50

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Horse, Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A fusion protein containing the first 33 amino acid residues from human CB2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2082910

Product Specific Information

PA1-744 detects cannabinoid receptor 2 (CB2) from human tissues.

PA1-744 has been successfully used in Western blot, FACS, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa band from human spinal cord homogenate, and a ~40 kda band from RBL2H3 cells, representing the different glycosylated forms of CB2.

PA1-744 antigen is a fusion protein containing the first 33 amino acid residues from human CB2.

Target Information

Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin.

Journal of veterinary internal medicine

Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

PA1-744 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 EDT 2022

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin.

Journal of veterinary internal medicine

Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

PA1-744 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 EDT 2022

Revisiting cannabinoid receptor 2 expression and function in murine retina.

Neuropharmacology

Borowska-Fielding J,Murataeva N,Smith B,Szczesniak AM,Leishman E,Daily L,Toguri JT,Hillard CJ,Romero J,Bradshaw H,Kelly MEM,Straiker A

PA1-744 was used in Immunohistochemistry-immunofluorescence to show minimal CB2 expression is seen in healthy retina and CB2 appears to be upregulated under pathological conditions.

Mon Oct 01 00:00:00 EDT 2018

Chemical Tools for Studying Lipid-Binding Class A G Protein-Coupled Receptors.

Pharmacological reviews

Cooper A,Singh S,Hook S,Tyndall JDA,Vernall AJ

PA1-744 was used in Flow cytometry/Cell sorting to review the radioligands, fluorescent ligands, covalent ligands, and antibodies that have been used to studying lipid-binding receptors.

Sat Jul 01 00:00:00 EDT 2017

Cannabinoid Receptors Are Overexpressed in CLL but of Limited Potential for Therapeutic Exploitation.

PloS one

Freund P,Porpaczy EA,Le T,Gruber M,Pausz C,Staber P,Jäger U,Vanura K

PA1-744 was used in western blot to study overexpression in cannabinoid receptors of CLL but a limited potential for therapeutic exploitation

Tue Jul 18 00:00:00 EDT 2017

Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.

Acta physiologica (Oxford, England)

Jean-Gilles L,Braitch M,Latif ML,Aram J,Fahey AJ,Edwards LJ,Robins RA,Tanasescu R,Tighe PJ,Gran B,Showe LC,Alexander SP,Chapman V,Kendall DA,Constantinescu CS

PA1-744 was used in flow cytometry and western blot to investigate the effects of proinflammatory cytokines on cannabinoid receptor expression and function

Fri May 01 00:00:00 EDT 2015

Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.

Acta physiologica (Oxford, England)

Jean-Gilles L,Braitch M,Latif ML,Aram J,Fahey AJ,Edwards LJ,Robins RA,Tanasescu R,Tighe PJ,Gran B,Showe LC,Alexander SP,Chapman V,Kendall DA,Constantinescu CS

PA1-744 was used in flow cytometry and western blot to investigate the effects of proinflammatory cytokines on cannabinoid receptor expression and function

Fri May 01 00:00:00 EDT 2015

Cannabinoid receptor CB2 is expressed on vascular cells, but not astroglial cells in the post-mortem human Huntington's disease brain.

Journal of chemical neuroanatomy

Dowie MJ,Grimsey NL,Hoffman T,Faull RL,Glass M

PA1-744 was used in immunocytochemistry and immunohistochemistry to study the expressino of cannabinoid receptor CB2 on vascular and astroglial cells in the post-mortem Huntington disease brain

Mon Sep 01 00:00:00 EDT 2014

Cannabinoids and bone: endocannabinoids modulate human osteoclast function in vitro.

British journal of pharmacology

Whyte LS,Ford L,Ridge SA,Cameron GA,Rogers MJ,Ross RA

PA1-744 was used in immunocytochemistry to investigate the effect of the endocannabinoids on human osteoclast function in vitro

Sun Apr 01 00:00:00 EDT 2012

The expression of the peripheral cannabinoid receptor CB2 has no effect on clinical outcome in diffuse large B-cell lymphomas.

European journal of haematology

Rayman N,Lam KH,van der Holt B,Koss C,van Leeuwen J,Budel LM,Mulder AH,Sonneveld P,Delwel R

PA1-744 was used in immunohistochemistry to study the influence of cannabinoid receptor CB2 expression on clinical outcomes of lymphomas

Wed Jun 01 00:00:00 EDT 2011

Cannabinoid receptors in human astroglial tumors.

Journal of neurochemistry

Held-Feindt J,Dörner L,Sahan G,Mehdorn HM,Mentlein R

Published figure using CNR2 polyclonal antibody (Product # PA1-744) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2006

Characterization of peripheral human cannabinoid receptor (hCB2) expression and pharmacology using a novel radioligand, [35S]Sch225336.

The Journal of biological chemistry

Gonsiorek W,Hesk D,Chen SC,Kinsley D,Fine JS,Jackson JV,Bober LA,Deno G,Bian H,Fossetta J,Lunn CA,Kozlowski JA,Lavey B,Piwinski J,Narula SK,Lundell DJ,Hipkin RW

PA1-744 was used in western blot to show that PA1-744 can not immunoreact with hCB2 successfully

Fri Sep 22 00:00:00 EDT 2006

A glial endogenous cannabinoid system is upregulated in the brains of macaques with simian immunodeficiency virus-induced encephalitis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Benito C,Kim WK,Chavarría I,Hillard CJ,Mackie K,Tolón RM,Williams K,Romero J

PA1-744 was used in immunohistochemistry to investigate the role of cannabinoid receptors during the development of human immunodeficiency virus-induced encephalitis.

Wed Mar 09 00:00:00 EST 2005

The peripheral cannabinoid receptor Cb2, frequently expressed on AML blasts, either induces a neutrophilic differentiation block or confers abnormal migration properties in a ligand-dependent manner.

Blood

Alberich Jordà M,Rayman N,Tas M,Verbakel SE,Battista N,van Lom K,Löwenberg B,Maccarrone M,Delwel R

PA1-744 was used in immunocytochemistry to study the mechanism of Cb2-mediated differentiation block.

Thu Jul 15 00:00:00 EDT 2004

Distinct expression profiles of the peripheral cannabinoid receptor in lymphoid tissues depending on receptor activation status.

Journal of immunology (Baltimore, Md. : 1950)

Rayman N,Lam KH,Laman JD,Simons PJ,Löwenberg B,Sonneveld P,Delwel R

PA1-744 was used in immunohistochemistry to study the role of CB2 in the migration and activation of B cells.

Sun Feb 15 00:00:00 EST 2004

Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Benito C,Núñez E,Tolón RM,Carrier EJ,Rábano A,Hillard CJ,Romero J

PA1-744 was used in immunohistochemistry to study the expression of cannabinoid CB2 receptors and fatty acid amide hydrolase in neuritic plaque-associated glia in Alzheimer disease brains.

Wed Dec 03 00:00:00 EST 2003

Bioinformatics

Protein Aliases: Cannabinoid Receptor 2; cannabinoid receptor 2 (macrophage); CB-2; CB2R; CNR3; CX5; HCB2; HGNC:2160; OTTHUMP00000044841; RP11-4M23.1; testis-dominant CNR2 isoform CB2

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Gene Aliases: CB-2; CB2; CB2A; CB2B; CNR2; CX5

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UniProt ID: (Human) P34972

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Entrez Gene ID: (Human) 1269

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Function(s)

cannabinoid receptor activity G-protein coupled receptor

Process(es)

response to amphetamine inflammatory response immune response G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger sensory perception of pain negative regulation of synaptic transmission, GABAergic response to lipopolysaccharide negative regulation of mast cell activation cannabinoid signaling pathway negative regulation of action potential negative regulation of inflammatory response negative regulation of nitric-oxide synthase activity

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CNR2 Polyclonal Antibody

CNR2 Antibody (PA1-744) in WB

Product Details

PA1-744

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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