Invitrogen

COL9A1 Polyclonal Antibody

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COL9A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of COL9A1 in paraffin-embedded human stomach using COL9A1 Polyclonal Antibody (Product # PA5-93062) at a dilution of 1:200.

COL9A1 Antibody in Western Blot (WB) — Western Blot analysis of COL9A1 in extracts of mouse skeletal muscle using COL9A1 Polyclonal Antibody (Product # PA5-93062) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

COL9A1 Antibody in Immunohistochemistry (IHC) — Figure 4. Proteins Regulated during Noise Exposure Are Expressed by HCs, SGNs, and Supporting Cells within the Cochlea (A) DAPI-stained midmodiolar cochlear section. (B) Col9a1 signal decreases in the area above the tunnel of Corti following noise exposure. Green, Col9a1; red, CtBP2. (C) Hsp90b1 levels are elevated in both IHCs and OHCs after exposure to increasing intensities of noise. Green, Hsp90b1; red, CtBP2; white, DAPI. (D) Psmc5 levels increase after exposure to increasing noise intensity within IHCs. Green, Psmc5; red, CtBP2. (E) Vcp levels are elevated in the OHCs after exposure to increasingly damaging levels of noise. Green, Vcp; red, CtBP2; white, DAPI. (F) SGNs from the second ganglion bundle from the top of the cochlea (12-16 kHz) in midmodiolar sections. Left: Arpc2 levels are elevated in SGNs after exposure to increasingly damaging levels of noise. Middle: Uba2 levels progressively increase within SGNs with increasing noise exposure. Right: LtBP4 levels are reduced in SGNs after exposure to increasingly damaging levels of noise. Green, Arpc2, Uba2, and LtBP4 (left, middle, and right); red, NF200. Scale bars, 100 mum (A) and 10 mum (B-F). Representative images from n = 2 animals.

Product Details

PA5-93062

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human COL9A1 View immunogen
Conjugate	Unconjugated
Form	liquid
Concentration	0.32 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2806609

Product Specific Information

Immunogen sequence: WASAAVKRRP RFPVNSNSNG GNELCPKIRI GQDDLPGFDL ISQFQVDKAA SRRAIQRVVG SATLQVAYKL GNNVDFRIPT RNLYPSGLPE EYSFLTTFRM TGSTLKKNWN IWQIQDSSGK EQVGIKINGQ TQSVVFSYKG LDGSLQTAAF SNLSSLFDSQ WHKIMIGVER SSATLFVDCN RIESLPIKPR GPIDIDGFAV LGKLADNPQV SVPFELQWML IHCDPLRPRR ETCHELPARI TPSQTTDERG P; Positive Samples: Mouse skeletal muscle; Cellular Location: extracellular matrix, extracellular space

Target Information

COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network.

Cell reports

Jongkamonwiwat N,Ramirez MA,Edassery S,Wong ACY,Yu J,Abbott T,Pak K,Ryan AF,Savas JN

Published figure using COL9A1 polyclonal antibody (Product # PA5-93062) in Immunohistochemistry

Tue Nov 24 00:00:00 EST 2020

Bioinformatics

Protein Aliases: alpha-1(IX) collagen chain; cartilage-specific short collagen; Collagen alpha-1(IX) chain; collagen IX, alpha-1 polypeptide; collagen, type IX, alpha 1; procollagen, type IX, alpha 1

Gene Aliases: COL9A1; DJ149L1.1.2; EDM6; MED; STL4

UniProt ID: (Human) P20849

Entrez Gene ID: (Human) 1297, (Rat) 305104

Molecular Function: extracellular matrix structural protein