Western blot analysis of EBS E14 TG2A Day 0 whole cell lysate using PA5-19850, COMP primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19850 staining of Raji whole cell lysate at a dilution of 1 ug/ml (Lane 2). PA5-19850 staining of HepG2 whole cell lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human COMP.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 65 kDa.
This antibody is predicted to react with rat, horse, cow, dog and chimpanzee based on sequence homology.
The protein encoded by this gene is a noncollagenous extracellular matrix protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia and multiple epiphyseal dysplasia .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.