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Invitrogen
COMT Polyclonal Antibody
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FIGURE: 1 / 4
COMT Antibody (PA5-76864) in WB
Western blot was performed using Anti-COMT Polyclonal Antibody (Product # PA5-76864) and 30kDa (M-COMT) and 24kDa (S-COMT) bands corresponding to the two forms of COMT were observed across the cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane2), PC-3 (Lane 3), THP-1 (Lane 4), Mouse Liver (Lane 5) and Mouse Adrenal Gland (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by ... View More
Product Details
PA5-76864
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human COMT.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Catechol O-methyltransferase; catechol-O-methyltransferase 1; COMT; epididymis secretory sperm binding protein Li 98n; OTTHUMP00000197749; OTTHUMP00000197750; testicular tissue protein Li 42; unnamed protein product
Gene Aliases: COMT; Comt1; D16Wsu103e; D330014B15Rik; HEL-S-98n
UniProt ID: (Human) P21964, (Mouse) O88587
Entrez Gene ID: (Human) 1312, (Mouse) 12846
behavioral fear response
response to hypoxia
kidney development
synaptic transmission, dopaminergic
startle response
response to amphetamine
renin secretion into blood stream
glycogen metabolic process
catecholamine metabolic process
lipid metabolic process
prostaglandin metabolic process
response to stress
response to oxidative stress
learning or memory
learning
memory
regulation of blood pressure
visual learning
response to temperature stimulus
response to xenobiotic stimulus
response to wounding
response to toxic substance
gene expression
dopamine secretion
cellular response to phosphate starvation
cerebellar cortex morphogenesis
response to food
methylation
developmental process
glomerulus development
cholesterol efflux
multicellular organismal response to stress
response to cytokine
multicellular organism growth
exploration behavior
renal sodium excretion
norepinephrine metabolic process
dopamine metabolic process
dopamine catabolic process
catecholamine catabolic process
fear response
habituation
norepinephrine secretion
cognition
detection of temperature stimulus involved in sensory perception of pain
response to corticosterone
artery development
cellular response to cocaine
mastication
renal albumin absorption
renal filtration
response to salt
response to dopamine
response to angiotensin
female pregnancy
short-term memory
estrogen metabolic process
catechol-containing compound metabolic process
negative regulation of renal sodium excretion
neurotransmitter catabolic process
negative regulation of dopamine metabolic process
response to pain
multicellular organismal reproductive process
negative regulation of smooth muscle cell proliferation
positive regulation of homocysteine metabolic process
regulation of sensory perception of pain
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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