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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • COPE Antibodies

          Invitrogen

          COPE Polyclonal Antibody

          View all (9) COPE antibodies

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          Cite COPE Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          COPE Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          COPE Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          COPE Antibody (PA5-76111) in WB

          Western blot analysis of COPE in extracts of various cell lines. Samples were incubated with COPE polyclonal antibody (Product # PA5-76111) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG at 1:10,000 dilution. Lysates/proteins: 25 µg per Lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          COPE Antibody in Western Blot (WB)
          COPE Antibody in Western Blot (WB)

          Product Details

          PA5-76111

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein of human COPE.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2719838

          Product Specific Information

          The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

          Target Information

          The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: coatomer epsilon subunit; coatomer protein complex, subunit epsilon; Coatomer subunit epsilon; epsilon coat protein; Epsilon-coat protein; Epsilon-COP; nonclathrin coat protein epsilon-COP

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          Gene Aliases: 1110005D17Rik; COPE; Cope1; epsilon-COP

          View more View less

          UniProt ID: (Human) O14579, (Mouse) O89079

          View more View less

          Entrez Gene ID: (Human) 11316, (Mouse) 59042, (Rat) 290659

          View more View less

          Function(s)
          structural molecule activity protein binding membrane traffic protein vesicle coat protein
          Process(es)
          ER to Golgi vesicle-mediated transport retrograde vesicle-mediated transport, Golgi to ER intra-Golgi vesicle-mediated transport protein transport transport vesicle-mediated transport
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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