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Invitrogen
COPE Polyclonal Antibody
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FIGURE: 1 / 8
COPE Antibody (PA5-79059) in ICC/IF
Immunocytochemistry analysis of COPE using anti-COPE antibody (Product # PA5-79059) . COPE was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-COPE antibody (Product # PA5-79059) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-79059
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human COPE recombinant protein (Position: E80-A308).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat stomach tissue, rat small intestine tissue, rat pancreas tissue, mouse stomach tissue, mouse small intestine tissue, mouse pancreas tissue, human MCF-7 whole cellm, human Hela whole cell, human 22RV1 whole cell. IHC: human mammary cancer tissue, mouse pancreas tissue, rat pancreas tissue. ICC/IF: U20S cell, MCF-7 cell. Flow: HepG2 cell.
Target Information
The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coatomer epsilon subunit; coatomer protein complex, subunit epsilon; Coatomer subunit epsilon; epsilon coat protein; Epsilon-coat protein; Epsilon-COP; nonclathrin coat protein epsilon-COP
Gene Aliases: 1110005D17Rik; COPE; Cope1; epsilon-COP
UniProt ID: (Human) O14579, (Mouse) O89079
Entrez Gene ID: (Human) 11316, (Mouse) 59042, (Rat) 290659
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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