Proteintech

COTL1 Polyclonal Antibody

View all (13) COTL1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (23)

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 23

COTL1 Antibody (10781-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 10781-1-AP) (COTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Western Blot (WB)

COTL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 10781-1-AP) (COTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10781-1-AP ( COTL1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10781-1-AP ( COTL1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 40x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 10x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 40x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 10x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 40x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 10x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 40x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 40x lens).

COTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 10781-1-AP (COTL1 antibody) at dilution of 1:50 (under 10x lens).

COTL1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10781-1-AP) COTL1 antibody at dilution of 1:8,000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10781-1-AP (COTL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

COTL1 Antibody in Immunoprecipitation (IP) — IP result of anti-COTL1 (IP:10781-1-AP, 3ug; Detection:10781-1-AP 1:3000) with mouse kidney tissue lysate 12500ug.

Product Details

10781-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COTL1 Fusion Protein Ag1230 (1-142 aa encoded by BC010884)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MATKIDKEA CRAAYNLVRD DGSAVIWVTF KYDGSTIVPG EQGAEYQHFI QQCTDDVRLF AFVRFTTGDA MSKRSKFALI TWIGENVSGL QRAKTGTDKT LVKEVVQNFA KEFVISDRKE LEEDFIKSEL KKAGGANYDA QTE (1-142 aa encoded by BC010884)

Target Information

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 UTC 2023

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 UTC 2023

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 UTC 2023

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 UTC 2023

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 UTC 2023

A Systemic and Integrated Analysis of p63-Driven Regulatory Networks in Mouse Oral Squamous Cell Carcinoma.

Cancers

Glathar AR,Oyelakin A,Nayak KB,Sosa J,Romano RA,Sinha S

Tue Jan 10 00:00:00 UTC 2023

Coactosin-Like Protein in Breast Carcinoma: Friend or Foe?

Journal of inflammation research

Wang B,Zhao L,Chen D

Tue Jul 26 00:00:00 UTC 2022

Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.

Experimental lung research

D'Anna C,Cigna D,Di Sano C,Di Vincenzo S,Dino P,Ferraro M,Bini L,Bianchi L,Di Gaudio F,Gjomarkaj M,Pace E

Fri Aug 17 00:00:00 UTC 2018

Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.

Experimental lung research

D'Anna C,Cigna D,Di Sano C,Di Vincenzo S,Dino P,Ferraro M,Bini L,Bianchi L,Di Gaudio F,Gjomarkaj M,Pace E

Fri Aug 17 00:00:00 UTC 2018

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Oncotarget

Guo S,Yang P,Jiang X,Li X,Wang Y,Zhang X,Sun B,Zhang Y,Jia Y

Tue Jan 03 00:00:00 UTC 2017

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Oncotarget

Guo S,Yang P,Jiang X,Li X,Wang Y,Zhang X,Sun B,Zhang Y,Jia Y

Tue Jan 03 00:00:00 UTC 2017

Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.

Biochimica et biophysica acta

Schröder M,Häfner AK,Hofmann B,Rådmark O,Tumulka F,Abele R,Dötsch V,Steinhilber D

Wed Oct 01 00:00:00 UTC 2014

Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.

Biochimica et biophysica acta

Schröder M,Häfner AK,Hofmann B,Rådmark O,Tumulka F,Abele R,Dötsch V,Steinhilber D

Wed Oct 01 00:00:00 UTC 2014

Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.

Journal of investigative medicine : the official publication of the American Federation for Clinical Research

Sun W,Guo C,Meng X,Yu Y,Jin Y,Tong D,Geng J,Huang Q,Qi J,Liu A,Guan R,Xu L,Sun D,Ji W,Liu P,Liu F,Sun H,Ji G,Fu S,Bai J

Sun Apr 01 00:00:00 UTC 2012

Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.

Journal of investigative medicine : the official publication of the American Federation for Clinical Research

Sun W,Guo C,Meng X,Yu Y,Jin Y,Tong D,Geng J,Huang Q,Qi J,Liu A,Guan R,Xu L,Sun D,Ji W,Liu P,Liu F,Sun H,Ji G,Fu S,Bai J

Sun Apr 01 00:00:00 UTC 2012

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

PloS one

Somalinga BR,Day CE,Wei S,Roth MG,Thomas PJ

Mon Sep 17 00:00:00 UTC 2012

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

PloS one

Somalinga BR,Day CE,Wei S,Roth MG,Thomas PJ

Mon Sep 17 00:00:00 UTC 2012

Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Journal of proteome research

Jeong HC,Kim GI,Cho SH,Lee KH,Ko JJ,Yang JH,Chung KH

Fri Jan 07 00:00:00 UTC 2011

Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Journal of proteome research

Jeong HC,Kim GI,Cho SH,Lee KH,Ko JJ,Yang JH,Chung KH

Fri Jan 07 00:00:00 UTC 2011

Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Journal of proteome research

Jeong HC,Kim GI,Cho SH,Lee KH,Ko JJ,Yang JH,Chung KH

Fri Jan 07 00:00:00 UTC 2011

Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Journal of proteome research

Jeong HC,Kim GI,Cho SH,Lee KH,Ko JJ,Yang JH,Chung KH

Fri Jan 07 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: coactosin-like 1; coactosin-like F-actin binding protein 1; Coactosin-like protein; coactosin-like protein {ECO:0000250|UniProtKB:Q14019}; cotl1 {ECO:0000312|EMBL:AAI58747.1, ECO:0000312|RGD:1305498}; FLJ43657; MGC19733

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Gene Aliases: 1810074P22Rik; 2010004C08Rik; CLP; COTL1

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UniProt ID: (Human) Q14019, (Mouse) Q9CQI6, (Rat) B0BNA5

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Entrez Gene ID: (Human) 23406, (Mouse) 72042, (Rat) 361422

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actin binding protein binding enzyme binding actin or actin-binding cytoskeletal protein cytoskeletal protein non-motor actin binding protein

Process(es)

biological_process defense response to fungus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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