Proteintech

COXIV Polyclonal Antibody

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COXIV Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 11242-1-AP (COXIV antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

COXIV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

COXIV Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COXIV Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with .2ug COXIV antibody (11242-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

COXIV Antibody in Immunoprecipitation (IP) — IP result of anti-COXIV (IP:11242-1-AP, 3ug; Detection:11242-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3500ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 66 publications 66 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COXIV Fusion Protein Ag1640

Conjugate

Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

Target Information

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4 (COX4I1) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cox iv; COX IV-1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; cytochrome c oxidase, subunit 4a; cytochrome c oxidase, subunit IV; cytochrome c oxidase, subunit IVa; FLJ23483; MGC72016

Gene Aliases: AL024441; COX; COX IV-1; COX4; COX4-1; Cox4a; COX4I1; COXIV; COXIV-1; IV-1

UniProt ID: (Human) Q6P666, (Rat) P10888, (Mouse) P19783

Entrez Gene ID: (Human) 1327, (Rat) 29445, (Mouse) 12857

Molecular Function: oxidase