Invitrogen

CPEB1 Polyclonal Antibody

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CPEB1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CPEB polyclonal antibody (Product # PA1-1100) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

CPEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CPEB polyclonal antibody (Product # PA1-1100) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

CPEB1 Antibody in Western Blot (WB) — Western blot analysis of CPEB was performed by loading 25 µg of Hela (lane 1), human brain (lane 2) and mouse brain (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a CPEB polyclonal antibody (Product # PA1-1100) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~62 kDa using an exposure time of 1 min (Hela) and 4 min (brain lysates).

Product Details

PA1-1100

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues C H(545) S M E G L R H H S P L M R N Q K N(562) of human CPEB. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2083772

Product Specific Information

PA1-1100 detects recombinant mouse and rat CPEB (cytoplasmic polyadenylation element binding protein).

PA1-1100 has been successfully used in Western blot, ICC/IF and IHC-P procedures. By Western blot, this antibody detects ~62 and kDa protein representing recombinant mouse CPEB.

The PA1-1100 immunogen is a synthetic peptide corresponding to residues C H(545) S M E G L R H H S P L M R N Q K N(562) of human CPEB. This sequence is completely conserved in mouse. The PA1-1100 immunizing peptide (Cat. # PEP-171) is available for use in neutralization and control experiments.

Target Information

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the nuclear membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the nuclear membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mammalian Oocytes Locally Remodel Follicular Architecture to Provide the Foundation for Germline-Soma Communication.

Current biology : CB

El-Hayek S,Yang Q,Abbassi L,FitzHarris G,Clarke HJ

PA1-1100 was used in Immunocytochemistry-immunoflourescence to show here in mice that these structures, termed transzonal projections, are specialized filopodia whose number amplifies enormously as oocytes grow, enabling increased germ-soma communication.

Mon Apr 02 00:00:00 EDT 2018

CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells.

Journal of cell science

Grudzien-Nogalska E,Reed BC,Rhoads RE

PA1-1100 was used in immunoprecipitation to study the mechanism by which knockdown of CPEB1 promotes mammary epithelial cell differentiation and blocks epithelial-to-mesenchymal transition

Thu May 15 00:00:00 EDT 2014

Time of day regulates subcellular trafficking, tripartite synaptic localization, and polyadenylation of the astrocytic Fabp7 mRNA.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Gerstner JR,Vanderheyden WM,LaVaute T,Westmark CJ,Rouhana L,Pack AI,Wickens M,Landry CF

PA1-1100 was used in immunoprecipitation to investigate the circadian control of astrocytic Fabp7 mRNA trafficking and subsequent translation

Wed Jan 25 00:00:00 EST 2012

Proteasomal activity is required to initiate and to sustain translational activation of messenger RNA encoding the stem-loop-binding protein during meiotic maturation in mice.

Biology of reproduction

Yang Q,Allard P,Huang M,Zhang W,Clarke HJ

PA1-1100 was used in western blot to test whether CPEB degradation is required for translational activation of Slbp mRNA

Fri Jan 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CEBP; CPE-binding protein 1; CPE-BP1; CPEB; CPEB-1; Cytoplasmic polyadenylation element-binding protein 1; FLJ13203; h-CEBP; mCPEB-1; MGC34136; MGC60106

Gene Aliases: AU024112; CPE-BP1; CPEB; CPEB-1; CPEB1; h-CPEB; hCPEB-1; mCPEB

UniProt ID: (Human) Q9BZB8, (Mouse) P70166, (Rat) P0C279

Entrez Gene ID: (Human) 64506, (Mouse) 12877, (Rat) 293056

Molecular Function: mRNA polyadenylation factor