Proteintech

CPN1 Polyclonal Antibody

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CPN1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of COLO 320 cells using 13385-1-AP (CPN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CPN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 40x lens).

CPN1 Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

CPN1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug CPN1 antibody (13385-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Product Details

13385-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CPN1 Fusion Protein Ag4035 (110-458 aa encoded by BC027897)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: RNQRIVQLI QDTRIHILPS MNPDGYEVAA AQGPNKPGYL VGRNNANGVD LNRNFPDLNT YIYYNEKYGG PNHHLPLPDN WKSQVEPETR AVIRWMHSFN FVLSANLHGG AVVANYPYDK SFEHRVRGVR RTASTPTPDD KLFQKLAKVY SYAHGWMFQG WNCGDYFPDG ITNGASWYSL SKGMQDFNYL HTNCFEITLE LSCDKFPPEE ELQREWLGNR EALIQFLEQV HQGIKGMVLD ENYNNLANAV ISVSGINHDV TSGDHGDYFR LLLPGIYTVS ATAPGYDPET VTVTVGPAEP TLVNFHLKRS IPQVSPVRRA PSRRHGVRAK VQPQARKKEM EMRQLQRGPA (110-458 aa encoded by BC027897)

Target Information

CPN1 is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The protein is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This protein is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High Expression of RAI14 in Triple-Negative Breast Cancer Participates in Immune Recruitment and Implies Poor Prognosis Through Bioinformatics Analyses.

Frontiers in pharmacology

Cui R,Zhao T,Bai C,Ji N,Hua J,Ren L,Li Y

Sat Jul 16 00:00:00 EDT 2022

Carboxypeptidase N1 is anticipated to be a synergy metrics for chemotherapy effectiveness and prognostic significance in invasive breast cancer.

Cancer cell international

Cui R,Wang C,Li T,Hua J,Zhao T,Ren L,Wang Y,Li Y

Thu Oct 28 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Anaphylatoxin inactivator; Arginine carboxypeptidase; carboxypeptidase K; Carboxypeptidase N catalytic chain; carboxypeptidase N catalytic subunit; Carboxypeptidase N polypeptide 1; carboxypeptidase N polypeptide 1 50 kD; Carboxypeptidase N small subunit; carboxypeptidase N, polypeptide 1; carboxypeptidase N, polypeptide 1, 50kD; CPN; FLJ40792; kininase I; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; SCPN; Serum carboxypeptidase N

Gene Aliases: 0610011F20Rik; ACBP; CPN; CPN1; SCPN

UniProt ID: (Human) P15169, (Rat) Q9EQV8, (Mouse) Q9JJN5

Entrez Gene ID: (Human) 1369, (Rat) 365466, (Mouse) 93721

Molecular Function: protease