Bethyl Laboratories

CPSF100 Polyclonal Antibody

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CPSF100 Antibody in Western Blot (WB) — **Detection of human and mouse CPSF100 by western blot (h and m) and immunoprecipitation (h).** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-CPSF100 antibody A301-583A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CPSF100 was also immunoprecipitated by rabbit anti-CPSF100 antibodies A301-581A and A301-582A, which recognize upstream epitopes. *Detection:* Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 732 and 782

Conjugate

Unconjugated

Form

liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

CPSF2 gene ontology annotations related to this gene include mRNA cleavage; mRNA polyadenylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cleavage and polyadenylation specific factor 2, 100kD subunit; cleavage and polyadenylation specific factor 2, 100kDa; Cleavage and polyadenylation specificity factor 100 kDa subunit; Cleavage and polyadenylation specificity factor subunit 2; CPSF 100 kDa subunit; CPSF 100kDa subunit

Gene Aliases: 100kDa; 2610024B04Rik; AI662483; Cpsf; CPSF100; CPSF2; KIAA1367; MCPSF; mKIAA1367

UniProt ID: (Human) Q9P2I0, (Mouse) O35218

Entrez Gene ID: (Human) 53981, (Mouse) 51786

Molecular Function: RNA binding protein endoribonuclease mRNA polyadenylation factor mRNA processing factor nuclease nucleic acid binding