Bethyl Laboratories

CPSF100 Polyclonal Antibody

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CPSF100 Antibody in Western Blot (WB) — **Detection of human and mouse CPSF100 by western blot (h&m) and immunoprecipitation (h).** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-CPSF100 antibody A301-583A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CPSF100 was also immunoprecipitated by rabbit anti-CPSF100 antibodies A301-581A and A301-582A, which recognize upstream epitopes. *Detection:* Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Product Details

A301-583A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunoprecipitation (IP)	2-5 µg/mg lysate	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 732 and 782
Conjugate	Unconjugated
Form	liquid
Concentration	0.20 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS with 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

CPSF2 gene ontology annotations related to this gene include mRNA cleavage; mRNA polyadenylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cleavage and polyadenylation specific factor 2, 100kD subunit; cleavage and polyadenylation specific factor 2, 100kDa; Cleavage and polyadenylation specificity factor 100 kDa subunit; Cleavage and polyadenylation specificity factor subunit 2; CPSF 100 kDa subunit; CPSF 100kDa subunit

Gene Aliases: 100kDa; 2610024B04Rik; AI662483; Cpsf; CPSF100; CPSF2; KIAA1367; MCPSF; mKIAA1367

UniProt ID: (Human) Q9P2I0, (Mouse) O35218

Entrez Gene ID: (Human) 53981, (Mouse) 51786

Function(s)

mRNA polyadenylation mRNA cleavage mRNA processing mRNA 3'-end processing by stem-loop binding and cleavage mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA export from nucleus mRNA 3'-end processing

Process(es)

RNA binding protein binding molecular_function