Recommended positive controls: 293T, A431, Raji.
Predicted reactivity: Mouse (98%), Rat (97%), Chicken (93%), Rhesus Monkey (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cleavage and polyadenylation specific factor 4, 30kDa; Cleavage and polyadenylation specificity factor 30 kDa subunit; Cleavage and polyadenylation specificity factor subunit 4; CPSF 30 kDa subunit; Neb-1; no; No arches homolog; no arches-like zinc finger protein; NS1 effector domain-binding protein 1
Gene Aliases: CPSF30; CPSF4; NAR; NEB-1; NEB1
UniProt ID: (Human) O95639
Entrez Gene ID: (Human) 10898