Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded cow lactate with a CPT1A polyclonal antibody (Product # PA5-12213) peroxidase-conjugated to the secondary antibody and followed by AEC staining (left) compared to a control with no antibody (right).
|Tested species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 606-636 amino acids from the C-terminal region of human CPT1A|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.