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Invitrogen
CR16 Polyclonal Antibody
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FIGURE: 1 / 2
CR16 Antibody (PA5-144045) in WB
Western blot analysis of WIPF3 using WIPF3 antibody (Product # PA5-144045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human K562 whole cell lysates; Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was ... View More
Product Details
PA5-144045
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human WIPF3 (EDFPPPDEYKPCQKIYPSKIPR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human 293T whole cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
May be a regulator of cytoskeletal organization. May have a role in spermatogenesis. Target protein binds SH3 domains; may play a role in MAP kinase mediated signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Corticosteroids and regional expression protein 16 homolog; FLJ36931; WAS/WASL-interacting protein family member 3
Gene Aliases: CR16; WIPF3
UniProt ID: (Human) A6NGB9
Entrez Gene ID: (Human) 644150
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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