Invitrogen

CRABP2 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CRABP2 Antibody in Immunocytochemistry (ICC/IF) — CRABP2 Polyclonal Antibody detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRABP2 protein stained by CRABP2 Polyclonal Antibody (Product # PA5-27451) diluted at 1:500. Blue: Hoechst 33342 staining.

CRABP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of CRABP2 was performed in paraffin-Embedded adult mouse retina tissue using Green: NF-H Polyclonal Antibody (Product # PA5-34759) at a dilution of 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

CRABP2 Antibody in Western Blot (WB) — Western Blot analysis of CRABP2 was performed by separating 50 µg of various tissue extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a CRABP2 Polyclonal Antibody (Product # PA5-27451) at a dilution of 1:1,000.

Product Details

PA5-27451

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 138 of Human CRABP2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544927

Product Specific Information

Recommended positive controls: A549, HepG2, mouse brain, mouse fetal brain, rat brain.

Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (89%), Pig (95%), Rhesus Monkey (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cellular retinoic acid binding protein (CRABP) is a low molecular weight protein with unknown function and existing in two isoforms, CRABP1 and CRABP2. The predicted amino acid sequences of human CRABP1 and CRABP2 exhibits 99.3 and 93.5% identity to mouse CRABP1 and CRABP2, respectively. The CRABP2 isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. The CRABP2 gene localized on human chromosome 1q21.3 is transcriptionally regulated by a newly synthesized regulatory protein. The protein is expressed in human adult liver.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status.

Nature communications

Coscia F,Watters KM,Curtis M,Eckert MA,Chiang CY,Tyanova S,Montag A,Lastra RR,Lengyel E,Mann M

PA5-27451 was used in immunohistochemistry - paraffin section and western blot determine the origin of human high-grade serous ovarian cancer cells

Fri Aug 26 00:00:00 EDT 2016

Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue.

Journal of the American Chemical Society

Chen W,Dong J,Plate L,Mortenson DE,Brighty GJ,Li S,Liu Y,Galmozzi A,Lee PS,Hulce JJ,Cravatt BF,Saez E,Powers ET,Wilson IA,Sharpless KB,Kelly JW

PA527451 was used in western blot to study the reactivity of arylfluorosulfates using the proteome of human cells as a substrate

Wed Jun 15 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: cellular lipid-binding protein; cellular retinoic acid binding protein II; Cellular retinoic acid-binding protein 2; Cellular retinoic acid-binding protein II; CRABP-II; RP11-66D17.4

Gene Aliases: AI893628; Crabp-2; CRABP-II; CRABP2; CrabpII; RBP6

UniProt ID: (Human) P29373, (Mouse) P22935, (Rat) P51673

Entrez Gene ID: (Human) 1382, (Mouse) 12904, (Rat) 29563

Function(s)

regulation of transcription, DNA-templated transport signal transduction epidermis development embryonic forelimb morphogenesis retinoic acid metabolic process regulation of retinoic acid receptor signaling pathway positive regulation of collateral sprouting retinoic acid biosynthetic process lipid transport

Process(es)

retinoic acid binding transporter activity retinoid binding protein binding retinal binding retinol binding lipid binding cyclin binding

CRABP2 Polyclonal Antibody

Product Details

PA5-27451

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!