Immunofluorescent analysis of CRABP2 showing staining in the cytoplasm and nucleus of HCT116 cells. HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CRABP2 polyclonal antibody (Product # PA5-27451) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 138 of Human CRABP2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
PA5-27451 targets CRABP2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27451 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 138 of Human CRABP2.
A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status.
PA5-27451 was used in immunohistochemistry - paraffin section and western blot determine the origin of human high-grade serous ovarian cancer cells
|Coscia F,Watters KM,Curtis M,Eckert MA,Chiang CY,Tyanova S,Montag A,Lastra RR,Lengyel E,Mann M||Nature communications (7:null)||2016|