Invitrogen

CRHR1 Polyclonal Antibody

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CRHR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence was performed on HEK-293 cells transfected with CRHR1 expression plasmid. 24 hours post transection, cells were fixed and permeabilized for detection of CRHR1 using Anti-CRHR1 Rabbit Polyclonal Antibody (Product # 720290, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CRHR1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Corticotropin releasing hormone receptor 1. Panel e) shows no signal in untransfected cells. Panel f) represents transfected cells with no primary antibody to assess background. The images were captured at 60X magnification.

CRHR1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg of lysate) of SH SY5Y (Lane1), MCF7 (Lane 2), NTERA-2 (Lane 3), NIH/3T3 (Lane 4), K-562 (Lane 5), HeLa (Lane 6), Mouse Cerebellum (Lane 7). The blots were probed with Corticotropin releasing hormone receptor 1 Rabbit Polyclonal Antibody (Product # 720290, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 47 kDa band corresponding to Corticotropin releasing hormone receptor 1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

720290

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to Human CRHR1 (aa 425-445)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2633242

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Pig, Rat and Mouse.

Target Information

Corticotropin-releasing factor receptor 1 (CRFR1/CRHR1), a Corticotropin-Releasing Factor Receptor, is involved in mediating the stress response and anxiety-related behaviors. Mutations of the receptor reduce anxiety-related behaviors and may be involved in the pathogenesis of Cushing disease. Multiple isoforms of CRFR1 are produced by alternative splicing. CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Type 1 Corticotropin-Releasing Factor Receptor Differentially Modulates Neurotransmitter Levels in the Nucleus Accumbens of Juvenile versus Adult Rats.

International journal of molecular sciences

Zegers-Delgado J,Aguilera-Soza A,Calderón F,Davidson H,Verbel-Vergara D,Yarur HE,Novoa J,Blanlot C,Bastias CP,Andrés ME,Gysling K

Published figure using CRHR1 polyclonal antibody (Product # 720290) in Western Blot

Fri Sep 16 00:00:00 EDT 2022

From the Cover: 2.45-GHz Microwave Radiation Impairs Hippocampal Learning and Spatial Memory: Involvement of Local Stress Mechanism-Induced Suppression of iGluR/ERK/CREB Signaling.

Toxicological sciences : an official journal of the Society of Toxicology

Shahin S,Banerjee S,Swarup V,Singh SP,Chaturvedi CM

720290 was used in Western Blotting to study the mechanisms responsible for microwave radiation induced impairment in memory formation.

Thu Feb 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Corticotropin-releasing factor receptor 1; corticotropin-releasing factor type 1 receptor; Corticotropin-releasing hormone receptor 1; CRF 1 receptor; Crf receptor 1; Crf type 1; CRF-R-1; CRF-R1; CRF-R1alpha; Crf1 receptor; CRFR-1; CRH receptor 1; CRH receptor subtype-1 isoform-3; CRH-R-1; CRH-R1; CRH-R1h; CRHR1f; seven transmembrane helix receptor

Gene Aliases: CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1; CRHR1L

UniProt ID: (Human) P34998, (Mouse) P35347

Entrez Gene ID: (Human) 1394, (Mouse) 12921

Molecular Function: transmembrane signal receptor