PA1-083 detects CRM1 (chromosome region maintenance 1) from bovine retinal extract, human and mouse samples.
PA1-083 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~135 kDa protein representing CRM1 from bovine retinal extract.
The PA1-083 immunogen is a synthetic peptide corresponding to residues C R(1043) Q A D E E K H K R Q M S V P G S(1058) of human CRM1. This sequence is 88% conserved in mouse, rat, and Xenopus laevis. The PA1-083 immunizing peptide (Cat. # PEP-162) is available for use in neutralization and control experiments.
Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: chromosome region maintenance 1 homolog; Chromosome region maintenance 1 protein homolog; Exp1; exportin 1 (CRM1 homolog, yeast); Exportin-1; exportin-1 (required for chromosome region maintenance)
Gene Aliases: CRM1; XPO1