Invitrogen

CRMP2 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

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CRMP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CRMP2 was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CRMP2 Rabbit Polyclonal Antibody (Product # PA5-29728) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CRMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of CRMP2 was performed in paraffin-embedded rat brain tissue using CRMP2 Polyclonal Antibody (Product # PA5-29728) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

CRMP2 Antibody in Western Blot (WB) — Western blot was performed using Anti-CRMP2 Polyclonal Antibody (Product # PA5-29728) and a 60 kDa band corresponding to CRMP2 was observed across all the cell lines tested except MCF-7 and SK-BR-3 which are reported to be negative. Whole cell extracts (30 µg lysate) of PC-12 (Lane 1), Neuro-2a (Lane 2), SH-SY5Y (Lane 3), U-87 MG (Lane 4), MCF-7 (Lane 5), MCF 10A (Lane 6), SK-BR-3 (Lane 7), tissue extracts of Mouse Brain (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CRMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CRMP2 was observed in PC-12, Neuro-2a, SH-SY5Y, U-87 MG, MCF 10A in comparison to MCF-7 and SK-BR-3 using Anti-CRMP2 Polyclonal Antibody (Product # PA5-29728) in Western Blot. {RE}

Product Details

PA5-29728

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:100,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 219 of Human CRMP2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.7 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547202

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (87%), Chicken (95%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Collapsin response mediator protein 2; collapsin response mediator protein hCRMP-2; CRMP-2; dihydropyrimidinase-like 2; Dihydropyrimidinase-related protein 2; DPYL2; DRP-2; N2A3; TOAD-64; Turned on after division 64 kDa protein; turned on after division, 64 kDa protein; ULIP-2; Unc-33-like phosphoprotein 2

Gene Aliases: AI851130; CRMP-2; CRMP2; DHPRP2; DPYSL2; DRP-2; DRP2; Musunc33; N2A3; TOAD-64; ULIP-2; ULIP2

UniProt ID: (Human) Q16555, (Rat) P47942, (Mouse) O08553

Entrez Gene ID: (Human) 1808, (Rat) 25416, (Mouse) 12934

Molecular Function: hydrolase