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CRTAC1 Antibody (PA5-95791) in WB
Western blot analysis of CRTAC1 in mouse brain. Samples were incubated with CRTAC1 Polyclonal antibody (Product # PA5-95791) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-95791
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 312-661 of human CRTAC1 (NP_0605283)
Conjugate
Form
Liquid
Concentration
2.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807593
Product Specific Information
Immunogen sequence: NWNGPHRLYL QMSTHGKVRF RDIASPKFSM PSPVRTVITA DFDNDQELEI FFNNIAYRSS SANRLFRVIR REHGDPLIEE LNPGDALEPE GRGTGGVVTD FDGDGMLDLI LSHGESMAQP LSVFRGNQGF NNNWLRVVPR TRFGAFARGA KVVLYTKKSG AHLRIIDGGS GYLCEMEPVA HFGLGKDEAS SVEVTWPDGK MVSRNVASGE MNSVLEILYP RDEDTLQDPA PLECGQGFSQ QENGHCMDTN ECIQFPFVCP RDKPVCVNTY GSYRCRTNKK CSRGYEPNED GTACVGTLGQ SPGPRPTTPT AAAATAAAAA AAGAATAAPV LVDGDLNLGS VVKESCEPSC; Positive Samples: Mouse brain; Cellular Location: extracellular matrix, extracellular space
Target Information
CRTAC1 encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa chondrocyte-expressed protein; ASPIC; Cartilage acidic protein 1; CEP-68; FLJ10320; Protein CRTAC1-B
Gene Aliases: 2810454P21Rik; Aspic1; AW047536; Cep68; Crtac1; Crtac1B; Lotus
UniProt ID: (Mouse) Q8R555
Entrez Gene ID: (Mouse) 72832
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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